Effects of oocyte‐derived paracrine factors on release of extracellular vesicles by murine mural granulosa cells in vitro
Effects of oocyte‐derived paracrine factors on release of extracellular vesicles by murine mural granulosa cells in vitro
复制标题
DOI：
10.1111/asj.13385
复制
发表时间：
2020-01
期刊：
影响因子：
--
通讯作者：
Yuta Matsuno;Natsumi Maruyama;W. Fujii;K. Naito;K. Sugiura
中科院分区：
文献类型：
--
作者：
Yuta Matsuno;Natsumi Maruyama;W. Fujii;K. Naito;K. Sugiura
卵母细胞和细胞外囊泡（EV）都是哺乳动物卵泡发育的关键调节因子；然而，卵母细胞衍生的旁分泌因子（ODPF）和EV信号之间可能的相互作用从未被研究过。因此，为了探索卵母细胞与EV信号相互作用的可能性，我们利用小鼠研究了ODPFs对EV生物发生的影响以及与EV生物发生相关的转录本在壁粒细胞（MGCs）中的表达水平。结果表明，虽然卵母细胞共培养对EV生物发生相关转录本的表达水平有一定影响，但条件培养基中存在的EV颗粒数量在ODPF处理和未处理的MGCs之间没有显著差异。因此，卵母细胞对MGCs的EV生物形成没有影响，至少在EV颗粒的数量方面没有影响。
Abstract Both oocytes and extracellular vesicles (EV) have emerged as critical regulators of mammalian follicular development; however, the possible interaction between the oocyte‐derived paracrine factor (ODPF) and EV signals has never been examined. Therefore, to explore the possibility of an interaction between oocyte and EV signals, the effects of ODPFs on the biogenesis of EVs as well as the expression levels of transcripts related to EV biogenesis in mural granulosa cells (MGCs) were examined using mice. The results showed that, while oocyte coculture has some effects on the expression levels of transcripts related to EV biogenesis, the number of EV particles present in the conditioned medium were not significantly different between ODPF‐treated and non‐treated MGCs. Therefore, oocytes have no effects on the EV biogenesis by MGCs, at least with respect to the numbers of EV particles.