Mapping of Ve in tomato:: a gene conferring resistance to the broad-spectrum pathogen, Verticillium dahliae race 1
Mapping of Ve in tomato:: a gene conferring resistance to the broad-spectrum pathogen, Verticillium dahliae race 1
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DOI：
10.1007/s001220051075
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发表时间：
1999-02-01
影响因子：
5.4
通讯作者：
Tanksley, SD
中科院分区：
文献类型：
--
作者：
Diwan, N;Fluhr, R;Tanksley, SD
土传真菌Verticillium spp.在许多农作物中引起维管枯萎病。在番茄中，对大丽轮枝菌生理小种1的抗性由一个显性基因Ve赋予。以前在番茄中绘制Ve的努力产生了令人困惑的结果，将其定位在不同的染色体上，随后提出了黄萎病抗性可能由许多基因座控制的可能性。我们使用了三个不同的映射群体，以获得一个明确的地图位置Ve：重组自交系（RI）的人口;分离的F-2人口的黄萎病抗性;和人口的50个渐渗系（IL）。在所有的作图群体中，Ve位于与RFLP标记GP 39紧密连锁的第9号染色体的短臂上。通过在已知对轮枝菌属的抗性或易感性的不同育种系中筛选GP 39来证实这种连锁。GP 39与这些品系的轮枝反应完全匹配，表明GP 39在快速检测轮枝抗性方面具有潜力，并可作为Ve.这种方法特别适用于大丽轮枝菌1号小种，因为在目前的工作中，我们还表明，感染番茄的分离物是其他重要作物枯萎病的原因。
The soil-borne fungi Verticillium spp. cause vascular wilt disease in a wide range of crop plants. In tomato, resistance to Verticillium dahliae race 1 is conferred by a single dominant gene, Ve. Previous efforts to map Ve in tomato have yielded confusing results, locating it on different chromosomes, which subsequently raised the possibility that Verticillium resistance may be controlled by a number of loci. We used three different mapping populations to obtain an unambiguous map location of Ve: a recombinant inbred (RI) line population; an F-2 population segregating for Verticillium resistance; and a population of 50 introgression lines (IL). In all of the mapping populations Ve was positioned on the short arm of chromosome 9 tightly linked to the RFLP marker GP39. This linkage was confirmed by screening for GP39 in different breeding lines with known resistance or susceptibility to Verticillium. A perfect match was found between GP39 and the Verticillium response of the lines, indicating the potential of GP39 in the rapid detection of Verticillium resistance and as a starting point for map-based cloning of Ve. This approach is particularly relevant for Verticillium dahliae race 1, since in the present work we also show that the isolate that infects tomato is responsible for wilt disease in other important crop plants.