Immunohistochemical analysis of human arrest-defective-1 expressed in cancers in vivo.
Immunohistochemical analysis of human arrest-defective-1 expressed in cancers in vivo.
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DOI：
10.3892/or_00000303
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发表时间：
2009-04
期刊：
影响因子：
4.2
通讯作者：
Min Yu;Junli Gong;Mingxing Ma;Hui Yang;J. Lai;Hong Wu;Lin Li;La-Xiu Li;D. Tan
中科院分区：
文献类型：
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作者：
Min Yu;Junli Gong;Mingxing Ma;Hui Yang;J. Lai;Hong Wu;Lin Li;La-Xiu Li;D. Tan
Arrest-deficient-1（ARD 1）基因在酵母细胞周期调控中具有重要作用，最近的研究表明，人ARD 1基因与癌细胞增殖有关。为探讨hARD 1蛋白在肿瘤组织中的表达模式，采用免疫组化方法检测了19种常见肿瘤400例和11种组织类型133例非癌组织中hARD 1的表达模式。hARD 1蛋白在膀胱癌、乳腺癌和宫颈癌组织中的阳性表达率分别为71.5%（15/20）、62.5%（30/48）和57.1%（8/14）。hARD 1在恶性肿瘤中的平均阳性率为52.3%，在非恶性肿瘤中的平均阳性率为31.5%，两者之间有显著性差异（p<0.005）。比较同一切片中不同区域的染色强度，与癌旁细胞相比，hARD 1蛋白在癌细胞中高度积累。乳腺癌和肠癌的阳性率明显高于相应的非癌组织（P<0.05和0.01）。这些发现表明hARD 1蛋白的积累可能与各种类型癌症的发生有关。
The arrest-defective-1 (ARD1) gene has been reported to be important in yeast cell cycle regulation, and recent studies have shown that human arrest-defective-1 (hARD1) is related to cancer cell proliferation. To investigate the expression pattern of hARD1 protein in cancer tissues, immunohistochemical analysis was performed to analyze the hARD1 expression pattern in 400 cases of 19 types of common cancer and 133 non-cancer samples from 11 tissue types. hARD1 protein was expressed extensively in cancer tissues including glandular carcinoma and squamous cancer, and the positive rate was 71.5% (15/20) in urinary bladder cancer, 62.5% (30/48) in breast cancer and 57.1% (8/14) in cervical carcinoma. The average hARD1-positive rate was 52.3% in cancers and 31.5% in non-cancers, for which the difference was significant (p<0.005). Comparing the staining intensity of different fields in the same section, the hARD1 protein was highly accumulated in cancer cells when compared to the cells adjacent to cancer. The positive rate of breast and intestinal cancer was obviously higher than corresponding non-cancers (p<0.05 and 0.01). These findings suggest that the accumulation of hARD1 protein may be related to carcinogenesis of various types of cancer.