Capillary electrophoresis of proteins and nucleic acids.
Capillary electrophoresis of proteins and nucleic acids.
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蛋白质和核酸的毛细管电泳。
DOI：
10.1146/annurev.bb.24.060195.003051
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Foret,F
中科院分区：
文献类型：
--
作者：
Karger,BL;Chu,YH;Foret,F
在过去的30年里，平板凝胶电泳已经成为现代生物化学、生物学和临床研究中最重要的工具之一。然而，尽管在方法学上取得了实质性的进步，平板凝胶技术通常存在着繁琐、耗时和难以自动化的问题。毛细管电泳法(CE)在十年前首次引入，现在作为平板凝胶技术的替代技术出现，具有现代自动化技术的所有优势。虽然毛细管电泳法的第一个目标是分析小分子(它是高效高效的高效替代品)，但现在主要关注的是生物聚合物。目前，CE可被视为一种用于快速、高灵敏度和定量分析微小(纳升)复杂样品的全自动化工具。本章回顾了最重要的毛细管电泳法及其在蛋白质和DNA分析和表征中的应用。
During the past 30 years, slab gel electrophoresis has been one of the most important tools available to modern biochemistry, biology, and clinical research. However, despite substantial progress in methodology, slab gel techniques typically suffer from laborious, time-consuming and difficult-to-automate procedures. Capillary electrophoresis (CE), first introduced a decade ago, emerges now as an alternative to slab gel techniques with all the advantages of modern automated technology. Although the first target of CE was analysis of small molecules (it is a highly efficient alternative to HPLC), now a main focus is on biopolymers. Currently, CE can be viewed as a fully automated tool for rapid, highly sensitive, and quantitative analysis of minute (nanoliter) amounts of complex samples. This chapter reviews the most important CE techniques and their use for the analysis and characterization of proteins and DNA.