Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature
Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature
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DOI：
10.1016/s0378-1119(97)00502-7
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发表时间：
1997-12-12
期刊：
影响因子：
3.5
通讯作者：
Hirai, A
中科院分区：
文献类型：
--
作者：
Ito, Y;Saisho, D;Hirai, A
我们从水稻中鉴定出了两个替代氧化酶（AOX）基因。一个 AOX 基因（命名为 AOX1a）位于大约。另一个 AOX 基因（称为 AOX1b）下游 1.9 kb。两个AOX基因的基因组和cDNA序列的比较表明，AOX1a基因被三个内含子中断，其他植物的AOX基因也是如此。另一方面，AOX1b 基因中插入了两个内含子。预测的 AOX1a 和 AOX1b 前体蛋白分别由 332 和 335 个氨基酸残基组成。基因组 Southern 杂交分析表明，除了两个串联排列的 A OX 基因外，水稻还具有多个 AOX 基因。 AOX1a 和 AOX1b 基因的稳态 mRNA 水平在低温（4 摄氏度）下均增加。然而，没有观察到 AOX1a 和 AOX1b 基因之间的转录诱导模式存在差异。 (C) 1997 Elsevier Science B.V.
We identified two genes for alternative oxidase (AOX) from rice. One AOX gene (designated AOX1a) is located approx. 1.9 kb downstream of another AOX gene (designated AOX1b). Comparison of the genomic and cDNA sequences of the two AOX genes showed that the AOX1a gene is interrupted by three introns, as are AOX genes of other plants. On the other hand, two introns are inserted in the AOX1b gene. The predicted AOX1a and AOX1b precursor proteins consist of 332 and 335 amino acid residues, respectively. A genomic Southern hybridization analysis indicated that rice has several AOX genes other than the two tandem-arranged A OX genes. Steady-state mRNA levels of both of the genes for AOX1a and AOX1b were increased under low temperature (4 degrees C). However, no difference in the pattern of induction of transcription between the genes for AOX1a and AOX1b was observed. (C) 1997 Elsevier Science B.V.