Impact of heterogeneity within cultured cells on bacterial invasion:: Analysis of Pseudomonas aeruginosa and Salmonella enterica serovar typhi entry into MDCK cells by using a green fluorescent protein-induced cystic fibrosis transmembrane conductance regulator receptor
Impact of heterogeneity within cultured cells on bacterial invasion:: Analysis of Pseudomonas aeruginosa and Salmonella enterica serovar typhi entry into MDCK cells by using a green fluorescent protein-induced cystic fibrosis transmembrane conductance regulator receptor
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DOI：
10.1128/iai.68.2.861-870.2000
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发表时间：
2000-02-01
影响因子：
3.1
通讯作者：
Pier, GB
中科院分区：
文献类型：
--
作者：
Gerçeker, AA;Zaidi, T;Pier, GB
囊性纤维化跨膜电导调节因子(CFTR)是一种氯离子通道，也是铜绿假单胞菌和伤寒沙门氏菌进入上皮细胞的受体。为了评价培养细胞中CFTR蛋白表达的异质性及其对不同铜绿假单胞菌和伤寒沙门氏菌内化的影响，我们利用双色流式细胞术和激光共聚焦显微镜研究了稳定表达绿色荧光蛋白(GFP)-CFTR融合结构的狗肾(MDCK)I型上皮细胞对细菌的摄取。我们发现细胞大小和GFP-CFTR蛋白的表达有很强的相关性，60%到70%的细胞表达低水平的GFP-CFTR蛋白，20%到30%的细胞表达中等水平的GFP-CFTR蛋白，以及
The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride ion channel that also serves as a receptor for entry of Pseudomonas aeruginosa and Salmonella enterica serovar Typhi into epithelial cells. To evaluate heterogeneity in CFTR protein expression in cultured cells and the effect of heterogeneity on internalization of different P. aeruginosa and serovar Typhi strains, we used two-color flow cytometry and confocal laser microscopy to study bacterial uptake by Madin-Darby canine kidney (MDCK) type I epithelial cells stably expressing a green fluorescent protein (GFP)-CFTR fusion construct (MDCK-GFP-CFTR cells). We found a strong correlation between cell size and GFP-CFTR protein expression, with 60 to 70% of cells expressing low levels of GFP-CFTR protein, 20 to 30% expressing intermediate levels, and