Fiberless multicolor neural optoelectrode for in vivo circuit analysis.
Fiberless multicolor neural optoelectrode for in vivo circuit analysis.
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DOI：
10.1038/srep30961
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发表时间：
2016-08-03
影响因子：
4.6
通讯作者：
Yoon E
中科院分区：
文献类型：
--
作者：
Kampasi K;Stark E;Seymour J;Na K;Winful HG;Buzsáki G;Wise KD;Yoon E
最大限度地发挥光遗传学方法在完整动物脑深部结构中的潜力，需要以高空间和时间分辨率对神经元进行光学操作，同时记录来自这些神经元的电数据。在这里，我们提出了第一个无光纤光电极与单片集成的光波导混频器，可以提供在一个共同的波导端口，以实现相同的神经元群体在体内的光调制。我们展示了成功的设备实现通过实现有效的耦合之间的侧发射注入激光二极管（ILD）和介电光波导混频器通过梯度折射率（GRIN）透镜。GRIN透镜的使用实现了几个设计特征，包括ILD和波导之间的高光学耦合和热隔离。我们在海马CA 1区锥体细胞中共表达视紫红质-2和古视紫红质的麻醉小鼠的完整脑中验证了包装的装置，实现了给定局部区域中完全相同的神经元的高质量记录、激活和沉默。这种完全集成的方法证明了空间精度和可扩展性，从而能够独立激活和沉默密集大脑区域中相同或不同的神经元组，同时记录它们，从而大大提高了目前可用的光遗传学工具集的能力。
Maximizing the potential of optogenetic approaches in deep brain structures of intact animals requires optical manipulation of neurons at high spatial and temporal resolutions, while simultaneously recording electrical data from those neurons. Here, we present the first fiber-less optoelectrode with a monolithically integrated optical waveguide mixer that can deliver multicolor light at a common waveguide port to achieve multicolor modulation of the same neuronal population in vivo. We demonstrate successful device implementation by achieving efficient coupling between a side-emitting injection laser diode (ILD) and a dielectric optical waveguide mixer via a gradient-index (GRIN) lens. The use of GRIN lenses attains several design features, including high optical coupling and thermal isolation between ILDs and waveguides. We validated the packaged devices in the intact brain of anesthetized mice co-expressing Channelrhodopsin-2 and Archaerhodopsin in pyramidal cells in the hippocampal CA1 region, achieving high quality recording, activation and silencing of the exact same neurons in a given local region. This fully-integrated approach demonstrates the spatial precision and scalability needed to enable independent activation and silencing of the same or different groups of neurons in dense brain regions while simultaneously recording from them, thus considerably advancing the capabilities of currently available optogenetic toolsets.