Anti-inflammatory Activity of Magnesium Isoglycyrrhizinate Through Inhibition of Phospholipase A2/Arachidonic Acid Pathway
Anti-inflammatory Activity of Magnesium Isoglycyrrhizinate Through Inhibition of Phospholipase A2/Arachidonic Acid Pathway
复制标题
异甘草酸镁通过抑制磷脂酶 A2/花生四烯酸途径的抗炎活性
DOI：
10.1007/s10753-015-0140-2
复制
发表时间：
2015-08-01
期刊：
影响因子：
5.1
通讯作者：
Zhong, Caiyun
中科院分区：
文献类型：
--
作者：
Xie, Chunfeng;Li, Xiaoting;Zhong, Caiyun
甘草具有抗炎、抗氧化、抗病毒、保肝等多种药理活性。异甘草酸镁（MgIG）是18-α-甘草酸立体异构体的镁盐，临床上用于治疗炎症性肝病。然而，MgIG发挥其抗炎作用的机制仍不清楚。在本研究中，我们研究了MgIG对磷脂酶A（2）（PLA（2））/花生四烯酸（AA）途径的抑制潜力以及对RAW 264. 7巨噬细胞释放途径产生的炎症脂质介质的抑制作用。结果显示，MgIG抑制LPS诱导的巨噬细胞PLA（2）活化和AA代谢产物如前列腺素E-2（PGE（2））、前列环素（PGI（2））、血栓素B2（TXB 2）和白三烯（LTB 4）的产生。此外，MgIG对LPS诱导的AA代谢酶包括考克斯-2、考克斯-1、5-LOX、TXB合成酶和PGI（2）合成酶均有明显抑制作用。总之，我们的数据表明，环氧化酶（COXs）和5-脂氧合酶（LOX）的AA代谢途径的调制可能是一种新的机制，镁IG的抗炎作用。
Glycyrrhiza glabra (licorice) has been known to possess various pharmacological properties including anti-inflammatory, antioxidants, antiviral, and hepatoprotective activities. Magnesium isoglycyrrhizinate (MgIG), a magnesium salt of 18-alpha glycyrrhizic acid stereoisomer, is clinically used for the treatment of inflammatory liver diseases. However, the mechanism by which MgIG exerts its anti-inflammatory effects remains unknown. In the present study, we investigated the inhibitory potential of MgIG in phospholipase A(2) (PLA(2))/arachidonic acid (AA) pathway and release of the pathway-generated inflammatory lipid mediators in RAW264.7 macrophages. Results revealed that MgIG suppressed LPS-induced activation of PLA(2) and production of AA metabolites such as prostaglandin E-2 (PGE(2)), prostacyclin (PGI(2)), thromboxane 2 (TXB2), and leukotrienes (LTB4) in macrophages. Furthermore, LPS-induced AA-metabolizing enzymes including COX-2, COX-1, 5-LOX, TXB synthase, and PGI(2) synthase were significantly inhibited by MgIG. Taken together, our data suggest that modulation of cyclooxygenase (COXs) and 5-lipoxygenase (LOX) pathways in AA metabolism could be a novel mechanism for the anti-inflammatory effects of MgIG.