Aldosterone and dexamethasone binding in human arterial smooth muscle cells.
Aldosterone and dexamethasone binding in human arterial smooth muscle cells.
复制标题
醛固酮和地塞米松在人动脉平滑肌细胞中的结合。
DOI：
10.1097/00004872-198712000-00018
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发表时间：
1987
影响因子：
4.9
通讯作者：
Meyer3rd,WJ
中科院分区：
文献类型：
--
作者：
Scott,BA;Lawrence,B;Nguyen,HH;Meyer3rd,WJ
1952年首次提出通过皮质类固醇对血管壁的直接作用来调节血压[1]。外周血管壁中存在这些激素的特异性受体是这一假说的先决条件。本文报道了醛固酮(AL)和地塞米松(DM)在培养的人动脉平滑肌细胞中的特异性结合。将培养的动脉平滑肌细胞与氚的AL或DM在37℃孵育30~60分钟后，超声处理细胞，Sephadex G25柱层析分离蛋白结合的类固醇组分。使用该组分的离子交换层析，每个皮质类固醇受体复合体都显示出不同的、可重现的洗脱模式。醛固酮在0.096+/-0.005摩尔/L磷酸钠处有一个单峰，糖尿病在0.029+/-0.003和0.050+/-0.004摩尔/L磷酸钠处有两个峰。由AL饱和曲线绘制的特异结合的Scatchard图为直线，平均+/-SD稳态结合参数为：解离常数(Kd)=0.35+/-0.15nmol/L，最大结合容量(Bmax)=98+/-53×10~(-18)mol/JJ-gDNA。Dm的平均+/-SD稳态结合参数为Kd=4.4+/-2.0nmol，Bmax=3031+/-1385×10~18mol/(Jig DNA)。因此，每个细胞大约有1150个AL结合位点和30000个DM结合位点。Kd值和Bmax值与先前描述的大鼠主动脉平滑肌细胞皮质激素受体的Kd值和Bmax值相似，并与生理类固醇浓度一致。动脉平滑肌细胞中存在特定的皮质类固醇受体支持这一假设，即这些类固醇直接参与人类血压的调节。
Regulation of blood pressure by direct action of corticosteroids on blood vessel walls was first hypothesized in 1952 [1]. The presence of receptors specific for these hormones within the peripheral vessel walls is a pre-requisite of this hypothesis. This report documents specific binding of both aldosterone (AL) and dexamethasone (DM) in cultured human arterial smooth muscle cells. After the arterial smooth muscle cultures were incubated with tritiated AL or DM at 37CC for 30-60 min, the cells were sonicated and the protein bound steroid fraction isolated on a Sephadex G25 column. Using ion exchange chromatography of this fraction, each corticosteroid receptor complex displayed a distinct, reproducible elution pattern. Aldosterone showed a single peak at 0.096+/-0.005 mol/l sodium phosphate and DM had two peaks at 0.029+/-0.003 and 0.050+/-0.004 mol/l sodium phosphate. The Scatchard plots of specific binding from AL saturation curves are linear and revealed mean+/-sd steady state binding parameters of dissociation constant (Kd)= 0.35+/-0.15 nmol/l and maximum binding capacity (Bmax)= 98+/-53 x 10~ 18 mol/jj-g DNA. Similarly, the mean+/-sd steady state binding parameters for DM are Kd= 4.4+/-2.0 nmol and Bmax= 3031+/-1385 x 10~ 18 mol/(jig DNA. Therefore, there are approximately 1150 AL binding sites and 30 000 DM binding sites per cell. The Kd and Bmax values are similar to those previously described for corticoid receptors in rat aortic smooth muscle cells and are consistent with physiological steroid concentrations. The presence of specific corticosteroid receptors in arterial smooth muscle cells supports the hypothesis that these steroids are directly involved in the regulation of blood pressure in humans.