Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis
Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis
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DOI：
10.1016/j.fertnstert.2007.12.033
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发表时间：
2008-06-01
影响因子：
6.7
通讯作者：
Yogev, Leah
中科院分区：
文献类型：
--
作者：
Kleiman, Sandra E.;Maymon, Batia Bar-Shira;Yogev, Leah
目的：测定混合睾丸组蛋白h4乙酰化及AZFc区受累情况。设计：前瞻性研究。单位：大学附属医疗中心。患者(S)：无精子症患者（n = 23），接受睾丸精子提取并准备进行卵胞浆内单精子注射（ICSI），分为精子发生完全的阻塞性无精子症（A组）、睾丸混合性萎缩（B组）和伴有AZFc缺失的睾丸混合性萎缩（C组）。干预(S)：睾丸活检通过免疫印迹和定量免疫组化评估组蛋白h4超乙酰化（Hypac-H4）和赖氨酸-12乙酰化（Lys12ac-H4）。主要观察指标：检索标本中被Hypac-H4和Lys12ac-H4抗体染色的精原细胞和精母细胞的百分比。结果(s): B组和C组精原细胞hdac - h4和Lys12ac-H4染色百分率均有统计学意义降低。B组Hypac-H4染色阳性的细长精子百分比比a组低，有统计学意义。各组中lys12ac - h4标记的精子百分比相似。Hypac-H4和Lys12ac-H4过程在精原细胞中高度相关，而在精母细胞中不相关。结论(5)：带C组含Hypac-H4和Lys12ac-H4的精原细胞比例降低可能与混合性萎缩精子产量降低有关。AZFc区域缺失后，混合性萎缩的精子Hypac-H4损伤没有进一步恶化。[j] .中国农业科学，2008;39(1):1 -3。(c) 2008年，美国生殖医学学会。)
Objective: To measure histone-H4 acetylation and involvement of the AZFc region in testicular mixed.Design: Prospective study.Setting: University-affiliated medical center.Patient(S): Azoospermic men (n = 23) who underwent testicular sperm extraction and preparation for intracytoplasmic sperm injection (ICSI) divided into obstructive azoospermia with complete spermatogenesis (group A), testicular mixed atrophy (group B), and testicular mixed atrophy associated with AZFc deletion (group C).Intervention(S): Testicular biopsy evaluation by Western blotting and quantitative immunohistochemistry of histone-H4 hyperacetylation (Hypac-H4) and lysine-12 acetylation (Lys12ac-H4).Main Outcome Measure(s): Percentage of spermatogonia and spermatids stained by Hypac-H4 and Lys12ac-H4 antibodies in retrieved specimens.Result(s): The percentage of spermatogonia stained for Hypac-H4 and Lys12ac-H4 in groups B and C was statistically significantly reduced. The percentage of elongated spermatids showing positive staining to Hypac-H4 was I statistically significantly lower in group B than group A. The percentage of Lys12ac-H4-labeled spermatids was similar for all groups. Hypac-H4 and Lys12ac-H4 processes were highly correlated in spermatogonia but not in spermatids.Conclusion(s): The reduced percentage of spermatogonia with Hypac-H4 and Lys12ac-H4 in groups Band C may contribute to lower sperm production in mixed atrophy. Spermatids Hypac-H4 impairment in mixed atrophy did not deteriorate further by AZFc region deletion. (Fertil Steril (R) 2008;89:1728-36. (c) 2008 by American Society for Reproductive Medicine.)