Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
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DOI：
10.4067/s0716-97602006000100004
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发表时间：
2006-01-01
影响因子：
6.7
通讯作者：
FCOLLINS, JAMES
中科院分区：
文献类型：
--
作者：
FCOLLINS, JAMES
Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during postnatal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duodenal and jejunal mucosa of three groups each of control and iron-deficient rats and hybridized with RAE 230A and 230B gene chips (Affymetrix). Stringent data reduction strategics were employed. Results showed that several genes were similarly induced in both gut segments, including DMIT1, Dcytb, transferrin receptor 1, heme oxygenase 1, metallothionein. the Menkes copper ATPase (ATP7A), tripartitic motif protein 27, and the sodium-dependent vitamin C transporter. However, a subset of genes showed regulation in only one or the other gut segment. In duodenum only, gastrokine 1, trefoil factor 1 and claudin 2 were induced by iron-deficiency. Other genes previously identified were only regulated in the duodenum. Overall, these studies demonstrate similarities and distinct differences in the genetic response to iron deprivation in the duodenum versus jejunum and provide evidence that more distal gut segments also may play a role in increasing iron absorption in iron-deficiency anemia.
