Effects of the stem cell factor, c-kit ligand, on human megakaryocytic cells.
Effects of the stem cell factor, c-kit ligand, on human megakaryocytic cells.
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DOI：
10.1182/blood.v79.2.365.bloodjournal792365
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发表时间：
1992
期刊：
影响因子：
20.3
通讯作者：
E. Vannier;Sally Cowley;Shuxian Jiang;S. Chi;C. Dinarello;K. Zsebo
中科院分区：
文献类型：
--
作者：
E. Vannier;Sally Cowley;Shuxian Jiang;S. Chi;C. Dinarello;K. Zsebo
Kit配体（KL），也称为干细胞因子（SCF），是最近发现的造血生长因子，其增强早期祖细胞对其他生长因子的反应并支持连续肥大细胞系的增殖。组织学研究表明，SCF/KL受体，c-kit原癌基因产物，存在于骨髓巨核细胞。我们研究了SCF/KL对永生化的人巨核细胞系（CMK，CMK 6和CMK 11 -5）和分离的人骨髓巨核细胞的影响。人SCF/KL单独或与造血生长因子、白细胞介素-3（IL-3）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和IL-6组合刺激这些巨核细胞系的增殖。SCF/KL处理未改变CMK细胞中gpIb、gpIIb/IIIa、LFA-1、ICAM-1或GMP-140的表达。未观察到对倍性的影响。此外，人SCF/KL诱导CMK细胞中IL-1 α、IL-1 β、IL-2和IL-6的表达。在纤维蛋白凝块系统中，SCF/KL单独加入含有CD 34+，DR+骨髓细胞的培养物中时，适度增强巨核细胞集落形成。加入SCF/KL和IL-3或GM-CSF后，骨髓巨核细胞数量明显增加。SCF/KL可直接影响巨核细胞的生成，也可通过诱导靶细胞产生细胞因子而间接调节造血。
The kit ligand (KL), also termed stem cell factor (SCF), is a recently discovered hematopoietic growth factor that augments response of early progenitor cells to other growth factors and supports proliferation of continuous mast cell lines. Histological studies suggest that the receptor for SCF/KL, the c-kit proto-oncogene product, is present in bone marrow megakaryocytes. We studied the effects of SCF/KL on immortalized human megakaryocytic cell lines (CMK, CMK6, and CMK11-5) and on isolated human marrow megakaryocytes. Human SCF/KL alone or in combination with the hematopoietic growth factors, interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-6, stimulated proliferation of these megakaryocytic cell lines. SCF/KL treatment did not alter expression of gpIb, gpIIb/IIIa, LFA-1, ICAM-1, or GMP-140 in CMK cells. No effect on ploidy was observed. Furthermore, human SCF/KL induced expression of IL-1 alpha, IL-1 beta, IL-2, and IL-6 in CMK cells. In a fibrin clot system, SCF/KL modestly potentiated megakaryocyte colony formation when added alone to cultures containing CD34+, DR+ bone marrow cells. Addition of SCF/KL with IL-3 or GM-CSF to these cultures resulted in a more marked marrow megakaryocytic cells. SCF/KL may directly affect megakaryocytopoiesis, as well as secondarily modulate hematopoiesis through induction of cytokines in target cells.