Analysis of translational products of Friend strain of spleen focus-forming virus
Analysis of translational products of Friend strain of spleen focus-forming virus
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脾病灶形成病毒Friend株转化产物分析
DOI：
10.1128/jvi.27.3.826-830.1978
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发表时间：
1978
影响因子：
5.4
通讯作者：
S. Aaronson
中科院分区：
文献类型：
--
作者：
M. Barbacid;D. Troxler;E. Scolnick;S. Aaronson
我们分析了正常大鼠肾细胞非生产性感染的朋友株脾病灶形成病毒（SFFV）的存在下，小鼠白血病病毒特异性C型病毒蛋白。通过抗原决定簇的免疫学分型，发现SFFV编码小鼠白血病病毒的p15和p12蛋白。p15和p12蛋白在SFFV nonproductively感染正常大鼠肾细胞的分子大小分析表明，这些蛋白被翻译为多蛋白分子的一部分。携带p12抗原决定簇的多肽的表观分子量揭示了SFFV基因组的翻译产物的存在，所述SFFV基因组的翻译产物不能被已知的C型病毒辅助结构蛋白解释。
We have analyzed normal rat kidney cells nonproductively infected with the Friend strain of spleen focus-forming virus (SFFV) for the presence of murine leukemia virus-specific type C viral proteins. SFFV was found to code for the p15 and p12 proteins of Friend-murine leukemia virus as determined by immunological typing of their antigenic determinants. Molecular-size analysis of p15 and p12 proteins in SFFV nonproductively infected normal rat kidney cells indicated that these proteins are translated as parts of polyprotein molecules. The apparent molecular weights of the polypeptides bearing p12 antigenic determinants revealed the presence of translational products of the SFFV genome that could not be accounted for by know type C virus helper structural proteins.