Production and consumption of hydrogen in hot spring microbial mats dominated by a filamentous anoxygenic photosynthetic bacterium.
Production and consumption of hydrogen in hot spring microbial mats dominated by a filamentous anoxygenic photosynthetic bacterium.
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DOI：
10.1264/jsme2.me11348
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发表时间：
2012
影响因子：
2.2
通讯作者：
Haruta S
中科院分区：
文献类型：
--
作者：
Otaki H;Everroad RC;Matsuura K;Haruta S
微生物垫含有丝状无氧光合细菌Chloroflexus aggregans发展在日本的Nakabusa温泉。在厌氧条件下，研究了硫酸盐还原菌和C。已提出聚集体作为硫化物消耗者构成硫循环;然而，在Nakabusa用于微生物硫化物生产的电子供体仍有待鉴定。为了确定这种电子供体及其来源，探索了垫的非原位实验孵育。在存在抑制生物硫酸盐还原的Na 2SO 4的情况下，氢气从垫样品中释放，表明这种氢通常作为电子供体被硫酸盐还原菌消耗。光照条件下，产氢量下降，表明C.聚集体还起到氢消耗者的作用。少量的氢也可能被消耗用于硫还原。克隆文库分析从垫扩增的16 S rRNA基因表明存在几种产氢发酵细菌。其中，最主要的发酵菌，热杆菌属，被成功隔离。该分离物通过有机碳发酵产生氢气。在垫中的微生物细胞的分散导致在不添加碳酸氢钠的情况下产生氢，这表明在垫中同时产生和消耗氢需要细胞的密集包装。我们提出了一个循环的电子流内的微生物垫，即，电子流通过三种元素发生：S（元素硫、硫化物、硫酸盐）、C（二氧化碳、有机碳）和H（二氢、质子）。
Microbial mats containing the filamentous anoxygenic photosynthetic bacterium Chloroflexus aggregans develop at Nakabusa hot spring in Japan. Under anaerobic conditions in these mats, interspecies interaction between sulfate-reducing bacteria as sulfide producers and C. aggregans as a sulfide consumer has been proposed to constitute a sulfur cycle; however, the electron donor utilized for microbial sulfide production at Nakabusa remains to be identified. In order to determine this electron donor and its source, ex situ experimental incubation of mats was explored. In the presence of molybdate, which inhibits biological sulfate reduction, hydrogen gas was released from mat samples, indicating that this hydrogen is normally consumed as an electron donor by sulfate-reducing bacteria. Hydrogen production decreased under illumination, indicating that C. aggregans also functions as a hydrogen consumer. Small amounts of hydrogen may have also been consumed for sulfur reduction. Clone library analysis of 16S rRNA genes amplified from the mats indicated the existence of several species of hydrogen-producing fermentative bacteria. Among them, the most dominant fermenter, Fervidobacterium sp., was successfully isolated. This isolate produced hydrogen through the fermentation of organic carbon. Dispersion of microbial cells in the mats resulted in hydrogen production without the addition of molybdate, suggesting that simultaneous production and consumption of hydrogen in the mats requires dense packing of cells. We propose a cyclic electron flow within the microbial mats, i.e., electron flow occurs through three elements: S (elemental sulfur, sulfide, sulfate), C (carbon dioxide, organic carbon) and H (di-hydrogen, protons).
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影响因子：
4.4
作者：
Kimura, Hiroyuki;Ishibashi, Jun-Ichiro;Hanada, Satoshi
通讯作者：
Hanada, Satoshi
影响因子：
2.8
作者：
Holkenbrink, Carina;Barbas, Santiago Ocon;Frigaard, Niels-Ulrik
通讯作者：
Frigaard, Niels-Ulrik
影响因子：
2.2
作者：
Everroad RC;Otaki H;Matsuura K;Haruta S
通讯作者：
Haruta S
影响因子：
2.8
作者：
MADIGAN, MT;PETERSEN, SR;BROCK, TD
通讯作者：
BROCK, TD
影响因子：
3.2
作者：
Hugenholtz, P;Pitulle, C;Pace, NR
通讯作者：
Pace, NR