Plasmodium berghei crystalloids contain multiple LCCL proteins
Plasmodium berghei crystalloids contain multiple LCCL proteins
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DOI：
10.1016/j.molbiopara.2009.11.008
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发表时间：
2010-03-01
影响因子：
1.5
通讯作者：
Dessens, Johannes T.
中科院分区：
文献类型：
--
作者：
Saeed, Sadia;Carter, Victoria;Dessens, Johannes T.
疟疾晶体是一种功能未知的独特细胞器，仅存在于蚊子特有的卵母细胞和寄生虫的早期卵囊阶段。最近，人们发现伯氏疟原虫的晶体形成与寄生虫蛋白PbSR有关，PbSR是疟原虫LCCL蛋白家族的一员，该家族由6个模块化多结构域蛋白组成，参与孢子子的发育和传染性。在这里，我们通过荧光蛋白标记表明，另外两个LCCL蛋白家族成员以类似于PbSR的方式靶向晶体。这些结果扩展了LCCL蛋白之间的相似性，并为该蛋白家族成员协同工作并参与类似分子过程的假设提供了强有力的支持证据。(C) 2009 Elsevier B.V.版权所有
Malaria crystalloids are unique organelles of unknown function that are present only in the mosquito-specific ookinete and early oocyst stages of the parasite. Recently, crystalloid formation in Plasmodium berghei was linked to the parasite protein PbSR, a member of the Plasmodium LCCL protein family composed of six modular multidomain proteins involved in sporozoite development and infectivity. Here, we show by fluorescent protein tagging that two other LCCL protein family members are targeted to the crystalloids in a similar way to PbSR. These results extend the similarities between the LCCL proteins, and provide strong supporting evidence for the hypothesis that members of this protein family work in concert and are involved in a similar molecular process. (C) 2009 Elsevier B.V. All rights reserved.