Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China.
Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China.
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DOI：
10.1590/s1517-83822012000200013
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发表时间：
2012-04
期刊：
影响因子：
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通讯作者：
Tongrige B
中科院分区：
文献类型：
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作者：
Huasai S;Chen A;Wang CJ;Li Y;Tongrige B
采用多重PCR和血清分型试验对健康奶牛分离的大肠杆菌毒力基因进行了鉴定和鉴定。结果表明，在101头奶牛的所有菌株中，检出率最高的是eaeA，占22.11% (67/303)；在22头牛中检出55株（18.15%）大肠杆菌，以aEPEC菌群分布最广。84株pcr阳性菌株共分为14个O型血清组，其中以O149（25.00%）最为常见，其次为O2（17.86%）、O8（11.90%）和O103(9.52%)，阳性率较高。
Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence.