Restoration of the subepidermal tissues and scale regeneration after wounding a cichlid fish, Hemichromis bimaculatus
Restoration of the subepidermal tissues and scale regeneration after wounding a cichlid fish, Hemichromis bimaculatus
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慈鲷 Hemichromis bimaculatus 受伤后表皮下组织的恢复和鳞片再生
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
J. Sire
中科院分区：
文献类型：
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作者：
A. Quilhac;J. Sire
利用鱼类的实验鳞片再生作为研究控制器官发生的表皮-真皮相互作用的一种手段。用光镜和电子显微镜观察了双斑双斑叶蝉的鳞片和表皮，并对鳞片的再生和修复进行了研究。此外，用氚胸腺嘧啶核苷掺入法研究了这些再生真皮组织中的细胞增殖。创面完全恢复了原有的鳞片模式，但与正常再生的外围区域相比，中央区域的恢复时间有所延迟。这种延迟是创面长期暴露于外界环境中的渗透性休克的结果，因为愈合的表皮对伤口的覆盖较晚。然而，渗透性休克并不像预期的那样严重，因为涉及到两种保护皮肤的手段：鳞片袋衬里(SPL)细胞起到抵抗渗透性休克的屏障作用，损伤组织产生的渗出物阻止水渗透。鳞片再生是在愈合的表皮与SPL细胞接触的每个鳞片袋中开始的，它向心性地从伤口的边缘向中心进行，表皮的愈合也是如此。再生鳞片的形状和大小反映了这种向心性的再上皮化，表明鳞片再生的第一个信号来自表皮。在迅速免受渗透休克的外围区域，鳞片在手术后几天内再生，而在中央区域，鳞片在能够再生之前必须恢复真皮，大约在手术后14天。真皮的愈合涉及经典的过程，包括成纤维细胞的迁移和增殖，随后是细胞外基质的沉积(主要是胶原)。在伤口中央形成的再生鳞片与正常的不同之处在于，由于SPL细胞群的破坏，新的鳞片形成细胞必须从未受伤的真皮周围区域招募。J.Exp.祖尔。281：305-327,1998。©1998 Wiley-Liss，Inc.
Experimental scale regeneration in fish is used as a means to study the epidermal-dermal interactions controlling organogenesis. Scales and epidermis were removed from a large surface area (1 cm2) of the flank in the cichlid Hemichromis bimaculatus, and scale regeneration and restoration of the subepidermal tissues were studied using light and electron microscopy. In addition, cell proliferation in these regenerating dermal tissues was studied using tritiated thymidine incorporation. The original squamation pattern was entirely restored in the wound region, but a delay was observed in the central region compared to the peripheral regions in which normal regeneration had occurred. This delay was the consequence of the osmotic shock in wound regions that were exposed to the external environment for a long time because of the late covering of the wound by the healing epidermis. However, the osmotic shock was not as severe as expected because two means of protection of the skin are involved: the scale-pocket lining (SPL) cells function as a barrier resisting the osmotic shock, and an exudate produced by the wounded tissues inhibits water penetration. Scale regeneration was initiated in each scale pocket where the healing epidermis entered in contact with the SPL cells, and it proceeded centripetally from the margins to the center of the wound, as did the healing of the epidermis. The shape and size of the regenerated scales reflected this centripetal re-epithelialization, suggesting that the first signal for scale regeneration came from the epidermis. In the peripheral regions, which were rapidly protected from osmotic shock, the scales regenerated in a few days, whereas in the central regions, the dermis had to be restored before scales were able to regenerate, approximately 14 days after surgery. Healing of the dermis involved classical processes including migration, and proliferation of fibroblast cells, followed by extracellular matrix deposition (mainly collagen). The regenerated scales formed in the central region of the wound differed from normal in that new scale-forming cells had to be recruited from the unwounded surrounding regions of the dermis, because of destruction of the SPL cell populations. J. Exp. Zool. 281:305–327, 1998. © 1998 Wiley-Liss, Inc.
影响因子：
4.6
作者：
M. Akimenko;Stephen L. Johnson;M. Westerfield;M. Ekker
通讯作者：
M. Akimenko;Stephen L. Johnson;M. Westerfield;M. Ekker