Influence of different types of exercise on the expression of haem oxygenase-1 in leukocytes
Influence of different types of exercise on the expression of haem oxygenase-1 in leukocytes
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不同运动类型对白细胞血红素加氧酶1表达的影响
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
H. Northoff
中科院分区：
文献类型：
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作者：
E. Fehrenbach;A. Nieß;F. Passek;S. Sorichter;A. Schwirtz;A. Berg;H. Dickhuth;H. Northoff
血红素加氧酶-1(HO-1)是一种抗氧化性应激蛋白，主要由活性氧、炎性细胞因子和高温诱导。我们观察了不同类型的运动对三组男性参与者外周血白细胞HO-1表达的影响：高于乳酸稳定状态的短程力竭跑(n = 15)、离心性运动(n = 12)和高强度耐力跑(半程马拉松，n = 12)。分别于安静时和运动后24小时采集血样。运动后血乳酸浓度力竭跑组为9.0 ± 2.1，离心性运动组为3.8 ± 1.6，半程马拉松组为5.1 ± 2.2 · L − 1( ± S)(P<0.0 5)。肌酸激酶浓度在运动后24 h最高，力竭组为133 ± 91，离心组为231 ± 139，半程马拉松组为289 ± 221U · L − 1(P <； 0.05)。运动后白细胞最大升高值分别为11.5 ± 19.2、6.2 ± 1.4和14.7 ± 2.1 × 109 · L − 1。短力竭跑和离心式运动对HO-1无明显影响，而半程马拉松对淋巴细胞、单核细胞和粒细胞HO-1表达有明显的刺激作用(P<；0.001)。总之，单独的离心性运动或短期大强度运动不足以刺激外周血白细胞中的抗氧化性应激蛋白HO-1
Haem-oxygenase-1 (HO-1) is an antioxidant stress protein that is mainly induced by reactive oxygen species, inflammatory cytokines and hyperthermia. We assessed the influence of different types of exercise on HO-1 expression in leukocytes of the peripheral blood in three groups of male participants: a short exhaustive run above the lactate steady state (n = 15), eccentric exercise (n = 12) and an intensive endurance run (half-marathon, n = 12). Blood samples were taken at rest and up to 24 h after exercise. Blood lactate concentration after exercise was 9.0 ± 2.1, 3.8 ± 1.6 and 5.1 ± 2.2 mmol · l − 1 (mean ± s) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). Creatine kinase concentration was highest 24 h after exercise: 133 ± 91, 231 ± 139 and 289 ± 221 U · l − 1 for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). The maximal increase in leukocyte counts after exercise was 11.5 ± 19.2, 6.2 ± 1.4 and 14.7 ± 2.1 × 109 · l − 1. There was no change in HO-1 as a result of the short exhaustive run or the eccentric exercise, whereas the half-marathon had a significant stimulatory effect on HO-1-expression in lymphocytes, monocytes and granulocytes (P<0.001) using flow cytometry analyses. In conclusion, eccentric exercise alone or short-term heavy exercise are not sufficient to stimulate the antioxidative stress protein HO-1 in peripheral leukocytes
DOI：
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发表时间：
1995
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
B. Vogt;J. Alam;A. J. Croatt;G. Vercellotti;K. Nath
通讯作者：
B. Vogt;J. Alam;A. J. Croatt;G. Vercellotti;K. Nath