Stratum Corneum Lipid Structure Investigated by EPR Spin-Probe Method : Application of Terpenes
Stratum Corneum Lipid Structure Investigated by EPR Spin-Probe Method : Application of Terpenes
复制标题
EPR旋转探针法研究角质层脂质结构：萜烯的应用
DOI：
10.1007/s11745-010-3479-z
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发表时间：
2010
期刊：
影响因子：
1.9
通讯作者：
K. Nakagawa and K. Anzai
中科院分区：
文献类型：
--
作者：
藤井智郁;山田耕輔;高橋正信;鈴木信雄;K. Nakagawa and K. Anzai
电子顺磁共振（EPR）结合缓慢翻滚模拟用于定义角质层（SC）脂质结构。无毛小鼠（HOS：HR-1）背部的SC使用涂有氰基丙烯酸酯树脂的玻璃板连续剥离1 - 3或4次。脂肪族自旋探针5-doxylstearic acid（5-DSA）和3β-doxyl-5α-cholestane（CHL）用于评价SC有序性。掺入SC中的5-DSA的EPR谱显示第一条带的特征峰。对5-DSA的慢速翻滚模拟显示，对照和萜烯处理的SC的EPR强度以及有序值（S 0）存在明显差异。α-松油醇对单链5-DSA的渗透促进作用约为对照的3倍。然而，在SC中的CHL的EPR光谱没有显示出明显的差异，为每个条带，除了信号强度。结果表明，CHL渗透到SC脂质不同于5-DSA。5-DSA的强化较CHL明显。因此，本结果可用于各种药物通过皮肤给药。
Electron paramagnetic resonance (EPR) in conjunction with a slow-tumbling simulation was utilized for defining stratum corneum (SC) lipid structure. SC from the back of hairless mouse (HOS:HR-1) was stripped consecutively from one to three or four times using a glass plate coated with a cyanoacrylate resin. Aliphatic spin probes, 5-doxylstearic acid (5-DSA) and 3β-doxyl-5α-cholestane (CHL), were used to evaluate the SC ordering. EPR spectrum of 5-DSA incorporated in the SC demonstrated a characteristic peak for the first strip. A slow-tumbling simulation for 5-DSA showed clear differences in EPR intensities as well as ordering values (S0) of the SC for control and terpenes treated SC. The α-terpineol enhanced the permeation of the single chain 5-DSA about three times more than that of the control. However, EPR spectra of CHL in the SC did not show a clear difference for each strip, except for the signal intensity. The results imply that CHL permeates into SC lipid differently from 5-DSA. The enhancement of the 5-DSA is more significant than that of CHL. Therefore, the present results can be useful for various drug administrations via the skin.