LncRNA colorectal neoplasia differentially expressed promotes glycolysis of liver cancer cells by regulating hypoxia-inducible factor 1α
LncRNA colorectal neoplasia differentially expressed promotes glycolysis of liver cancer cells by regulating hypoxia-inducible factor 1α
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DOI：
10.4103/0304-4920.365458
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发表时间：
2022-11
影响因子：
1.8
通讯作者：
Dan Tang;Lijin Zhao;Ruiz Mu;Yu Ao;Xuyang Zhang;Xiongxiong Li
中科院分区：
文献类型：
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作者：
Dan Tang;Lijin Zhao;Ruiz Mu;Yu Ao;Xuyang Zhang;Xiongxiong Li
LncRNA与肝癌的肿瘤发生相关。大肠肿瘤差异表达（CRNDE）是一种致癌的lncRNA，与肿瘤的生长和转移有关。研究CRNDE在肝癌中的作用。CRNDE在肝癌细胞中升高。CRNDE的敲低降低了细胞活力并抑制了肝癌的增殖。此外，CRNDE的敲低降低了细胞外酸化速率、葡萄糖消耗和乳酸产生的水平，从而抑制了肝癌的糖酵解。沉默CRNDE可增强miR-142的表达，降低zeste同源物2增强子（EZH 2）和缺氧诱导因子1α（HIF-1α）的表达。HIF-1α过表达可减弱CRNDE沉默诱导的葡萄糖消耗和乳酸产生减少。注射sh-CRNDE病毒通过上调miR-142、下调EZH 2和HIF-1α抑制肝癌体内肿瘤生长。总之，CRNDE的敲低通过EZH 2/miR-142/HIF-1α抑制肝癌的细胞增殖、糖酵解和肿瘤生长。
LncRNAs are associated with tumorigenesis of liver cancer. LncRNA Colorectal Neoplasia Differentially Expressed (CRNDE) was identified as an oncogenic lncRNA and involved in tumor growth and metastasis. The role of CRNDE in liver cancer was investigated. CRNDE was elevated in liver cancer cells. Knockdown of CRNDE decreased cell viability and inhibited proliferation of liver cancer. Moreover, knockdown of CRNDE reduced levels of extracellular acidification rate, glucose consumption, and lactate production to repress glycolysis of liver cancer. Silence of CRNDE enhanced the expression of miR-142 and reduced enhancer of zeste homolog 2 (EZH2) and hypoxia-inducible factor 1α (HIF-1α). Over-expression of HIF-1α attenuated CRNDE silence-induced decrease of glucose consumption and lactate production. Injection with sh-CRNDE virus reduced in vivo tumor growth of liver cancer through up-regulation of miR-142 and down-regulation of EZH2 and HIF-1α. In conclusion, knockdown of CRNDE suppressed cell proliferation, glycolysis, and tumor growth of liver cancer through EZH2/miR-142/HIF-1α.