Garlic revisited: antimicrobial activity of allicin-containing garlic extracts against Burkholderia cepacia complex.
Garlic revisited: antimicrobial activity of allicin-containing garlic extracts against Burkholderia cepacia complex.
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DOI：
10.1371/journal.pone.0112726
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Campopiano DJ
中科院分区：
文献类型：
--
作者：
Wallock-Richards D;Doherty CJ;Doherty L;Clarke DJ;Place M;Govan JR;Campopiano DJ
大蒜和其他植物葱的抗菌活性主要基于大蒜素，一种存在于压碎的大蒜鳞茎中的硫代亚硫酸盐。我们着手确定纯大蒜素和大蒜水提取物（AGE）是否对洋葱伯克霍尔德氏菌复合体（BCC）表现出抗菌特性，洋葱伯克霍尔德氏菌复合体是大蒜的主要细菌植物病原体，也是一种内在的多重耐药性和威胁生命的人类病原体。我们从市售大蒜鳞茎中制备AGE，并使用水性大蒜素标准（AAS）使用HPLC定量其中的大蒜素的量。最初，我们确定的最低抑菌浓度（MIC）的AGE对38 BCC分离株，这些MIC范围从0.5%至3%（v/v）。纯大蒜素（AAS）的抗微生物活性通过MIC和最小杀菌浓度（MBC）测定法对较小的五个BCC分离株进行了确认;这些分离株包括临床上最重要的物种B的三种代表性菌株。cenocepacia。在10倍MIC的条件下，时间杀菌试验表明AGE和AAS对B. B.新洋葱C6433与大蒜素浓度相关。我们还使用蛋白质质谱分析开始研究大蒜素与来自B的巯基依赖性过氧化物氧还蛋白（BCP，Prx）的重组形式的可能的分子机制。cenocepacia。这表明，AAS和AGE修改了一个重要的BCP催化半胱氨酸残基，并建议大蒜素作为一个通用的亲电试剂，目标蛋白质硫醇的作用。据我们所知，我们报告的第一个证据表明，大蒜素和大蒜提取物具有抑制和杀菌活性对BCC。目前针对这些威胁生命的病原体的治疗选择是有限的，因此，含有大蒜素的化合物值得研究作为现有抗生素的替代物。
The antimicrobial activities of garlic and other plant alliums are primarily based on allicin, a thiosulphinate present in crushed garlic bulbs. We set out to determine if pure allicin and aqueous garlic extracts (AGE) exhibit antimicrobial properties against the Burkholderia cepacia complex (Bcc), the major bacterial phytopathogen for alliums and an intrinsically multiresistant and life-threatening human pathogen. We prepared an AGE from commercial garlic bulbs and used HPLC to quantify the amount of allicin therein using an aqueous allicin standard (AAS). Initially we determined the minimum inhibitory concentrations (MICs) of the AGE against 38 Bcc isolates; these MICs ranged from 0.5 to 3% (v/v). The antimicrobial activity of pure allicin (AAS) was confirmed by MIC and minimum bactericidal concentration (MBC) assays against a smaller panel of five Bcc isolates; these included three representative strains of the most clinically important species, B. cenocepacia. Time kill assays, in the presence of ten times MIC, showed that the bactericidal activity of AGE and AAS against B. cenocepacia C6433 correlated with the concentration of allicin. We also used protein mass spectrometry analysis to begin to investigate the possible molecular mechanisms of allicin with a recombinant form of a thiol-dependent peroxiredoxin (BCP, Prx) from B. cenocepacia. This revealed that AAS and AGE modifies an essential BCP catalytic cysteine residue and suggests a role for allicin as a general electrophilic reagent that targets protein thiols. To our knowledge, we report the first evidence that allicin and allicin-containing garlic extracts possess inhibitory and bactericidal activities against the Bcc. Present therapeutic options against these life-threatening pathogens are limited; thus, allicin-containing compounds merit investigation as adjuncts to existing antibiotics.
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影响因子：
2.9
作者：
Clarke, David J.;Ortega, Ximena P.;Brown, Alan R.
通讯作者：
Brown, Alan R.
DOI：
10.1126/science.1176667
发表时间：
2009-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
Walsh CT
影响因子：
5.7
作者：
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通讯作者：
Jones A
影响因子：
1.6
作者：
Fujisawa, Hiroyuki;Watanabe, Kazuhiro;Ariga, Toyohiko
通讯作者：
Ariga, Toyohiko
影响因子：
4.9
作者：
Jakobsen, Tim Holm;van Gennip, Maria;Givskov, Michael
通讯作者：
Givskov, Michael