On-line precolumn enrichment of bisphenol A using boronate column in microcolumn liquid chromatography.
On-line precolumn enrichment of bisphenol A using boronate column in microcolumn liquid chromatography.
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DOI：
10.1016/j.chroma.2005.09.003
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发表时间：
2006-02
期刊：
影响因子：
--
通讯作者：
L. Lim;T. Takeuchi
中科院分区：
文献类型：
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作者：
L. Lim;T. Takeuchi
采用微柱液相色谱 (LC) 研究了硼酸盐预柱在痕量测定水溶液中 4,4'-异丙叉二酚（双酚 A 或 BPA）中的应用。通过填充 TSKgelboronate 的预柱富集 BPA，然后将其注入（反冲）至 ODS 分离柱。与传统注射器进样相比，硼酸盐柱前进样获得了更好的峰形，计算出的理论塔板数提高了大约两倍。保留时间、峰面积和峰高的相对标准偏差分别为 0.12、3.0 和 1.8%。该系统在工作样品浓度和样品体积范围内呈线性（R2>0.99），富集0.2mL样品时，检测限为0.09μgL−1（S/N=3）。该方法的回收率在107%至123%之间，该方法令人满意地应用于市售瓶装饮料样品中BPA的测定。事实证明，就水样中 BPA 的富集而言，硼酸盐预柱比 ODS 或 C30 预柱具有更好的灵敏度。
The application of a boronate precolumn in the trace determination of 4,4′-isopropylidenediphenol (bisphenol A or BPA) in aqueous solution was investigated by microcolumn liquid chromatography (LC). BPA was enriched by a precolumn packed with TSKgelboronate and it was then injected (back-flushed) into an ODS separation column. Comparing to a conventional syringe injection, a better peak shape was obtained via the boronate precolumn injection, and the calculated theoretical plate number improvement was approximately two folds. The relative standard deviations for the retention time, peak area and peak height were 0.12, 3.0 and 1.8%, respectively. The system is linear (R2>0.99) within the working sample concentration and sample-volume ranges, and it presents a detection limit of 0.09μgL−1(S/N=3) when a 0.2mL sample was enriched. Recovery values of the proposed method were between 107 and 123%, and this method was applied satisfactorily to the determination of BPA in commercially available bottled drink samples. The boronate precolumn was proven to produce better sensitivity than that of an ODS or C30precolumn as long as the enrichment of BPA in aqueous samples is concerned.