A novel glucose biosensor using bi-enzyme incorporated with peptide nanotubes.
A novel glucose biosensor using bi-enzyme incorporated with peptide nanotubes.
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DOI：
10.1016/j.bios.2012.06.005
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发表时间：
2012-10
影响因子：
12.6
通讯作者：
Byung-Wook Park;Rui Zheng;Kyoung-A Ko;B. Cameron;D. Yoon;Dong-Shik Kim
中科院分区：
文献类型：
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作者：
Byung-Wook Park;Rui Zheng;Kyoung-A Ko;B. Cameron;D. Yoon;Dong-Shik Kim
利用仿生肽纳米管（PNT）作为酶的包封模板，研制了一种新型的电流型葡萄糖生物传感器。用PNT包封辣根过氧化物酶（HRP），葡萄糖氧化酶（GOx）与PNT共固定在纳米金修饰的电极上。在电极表面形成由3-巯基丙酸（MPA）和1-十四硫醇（TDT）组成的二元SAM来固定PNT和GOx。所得到的电极似乎为酶提供了一个生物相容的纳米环境，因为它在较长时间内维持了增强的酶活性，并促进了通过PNT到电极的可能的直接电子转移。根据检测限、灵敏度、pH值、响应时间、选择性、再现性和实验室环境稳定性对生物传感器的性能进行了评估。此外，该传感器还对实际样品进行了测试。本研究采用AuNP-SAM-PNT/ hrp - gox复合材料制备传感器电极，在0.5 ~ 2.4mM的浓度范围内与葡萄糖呈线性关系，r2值为0.994。最大灵敏度为0.3mAM−1，重现性（RSD）为1.95%。储存一个月后，pnt包封酶的保留率为初始电流响应的85%。
A novel amperometric glucose biosensor was developed using the bio-inspired peptide nanotube (PNT) as an encapsulation template for enzymes. Horseradish peroxidase (HRP) was encapsulated by the PNT and glucose oxidase (GOx) was co-immobilized with the PNT on a gold nanoparticle (AuNP)-modified electrode. A binary SAM of 3-mercaptopropionic acid (MPA) and 1-tetradecanethiol (TDT) was formed on the surface of the electrode to immobilize the PNT and GOx. The resulting electrode appeared to provide the enzymes with a biocompatible nanoenvironment as it sustained the enhanced enzyme activity for an extended time and promoted possible direct electron transfer through the PNT to the electrode. Performance of the biosensor was evaluated in terms of its detection limit, sensitivity, pH, response time, selectivity, reproducibility, and stability in a lab setting. In addition the sensor was tested for real samples. The composite of AuNP-SAM-PNT/HRP-GOxto fabricate a sensor electrode in this study exhibited a linear response with glucose in the concentration range of 0.5−2.4mM with a R2-value of 0.994. A maximum sensitivity of 0.3mAM−1and reproducibility (RSD) of 1.95% were demonstrated. The PNT-encapsulated enzyme showed its retention of >85% of the initial current response after one month of storage.