Optimization of solid-phase extraction for a liquid chromatographic-tandem mass spectrometric general unknown screening procedure by means of computational techniques.
Optimization of solid-phase extraction for a liquid chromatographic-tandem mass spectrometric general unknown screening procedure by means of computational techniques.
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DOI：
10.1016/j.chroma.2004.06.010
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发表时间：
2004-11
期刊：
影响因子：
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通讯作者：
T. Decaestecker;W. Lambert;C. V. Van Peteghem;D. Deforce;J. Van Bocxlaer
中科院分区：
文献类型：
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作者：
T. Decaestecker;W. Lambert;C. V. Van Peteghem;D. Deforce;J. Van Bocxlaer
固相萃取（SPE）方法优化，以适应信息依赖获取LC-MS-MS程序在法医毒理学设置一般未知筛选的特殊要求。在第一阶段，进行了具有折叠的Plackett-Burman筛选设计，以区分影响提取过程的重要因素。这部分最终只确定了三个统计上相关的参数，需要连续优化。为此，在本研究的第二阶段，采用可旋转中心复合设计来定义响应面作为重要参数的函数，并选择SPE的最佳条件。
A solid-phase extraction (SPE) method was optimized to suit the particular demands of an information-dependent acquisition LC–MS–MS procedure for general unknown screening in a forensic toxicology setting. In a first phase, a Plackett–Burman screening design with fold-over was carried out to distinguish the significant factors affecting the extraction procedure. This part eventuated in the determination of only three statistically relevant parameters, requiring consecutive optimization. To that end, in phase II of this study, a rotatable central composite design was applied to define the response surface as a function of the significant parameters and to choose the optimal conditions for the SPE.