Flexible change and cooperation between mitochondrial electron transport and cytosolic glycolysis as the basis for chilling tolerance in tomato plants
Flexible change and cooperation between mitochondrial electron transport and cytosolic glycolysis as the basis for chilling tolerance in tomato plants
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线粒体电子传递与胞质糖酵解的灵活变化与配合是番茄植株耐冷性的基础
DOI：
10.1007/s00425-012-1799-3
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发表时间：
2012-11
期刊：
影响因子：
4.3
通讯作者：
Chen, Zhi-Xiang
中科院分区：
文献类型：
--
作者：
Yu, Jing-Quan;Fu, Li-Jun;Zhang, Shuai;Li, Xin;Liao, Yang-Wen-Ke;Xia, Xiao-Jian;Zhou, Yan-Hong;Wang, Rong-Qing;Chen, Zhi-Xiang
为了了解胞质糖酵解动态代谢是否在介导呼吸稳态中发挥作用及其与线粒体电子传递链（miETC）灵活性的关系，我们选择了两种耐冷性不同的番茄基因型，并比较了miETC、胞质糖酵解和呼吸的反应7 °C下的稳态。我们的结果表明，当在25 °C下生长时，两种基因型的miETC和糖酵解的经典和旁路组分基因的转录物相当。然而，两种基因型的大多数呼吸基因的表达在7 °C的低温下都迅速增加。以正常生长的植株为对照，耐冷的浙粉208植株中COX家族成员、ATP合成酶、AOX 1b和UCP的转录水平均比敏感的浙粉212植株高。这两种基因型都启动了蔗糖合成酶途径，通过胞质糖酵解降解蔗糖，但这一机制在抗性植株浙粉208中更为有效。此外，只有浙粉208号植株能够部分地从低能效途径转换到ATP保存途径来进行果糖-6-磷酸转化和丙酮酸生产。miETC和胞质糖酵解中的这种代谢灵活性与较高的ATP合成和较低的ROS积累相结合，这可能是维持耐冷植物较高的叶呼吸和体内平衡所必需的。
To find if cytosolic glycolysis dynamical metabolism plays a role in mediating respiration homeostasis and its relationship with mitochondrial electron transport chain (miETC) flexibility, we selected two tomato genotypes that differ in chilling tolerance and compared the responses of miETC, cytosolic glycolysis and respiratory homeostasis at 7 °C. Our results showed that the transcripts of both classical and bypass component genes for miETC and glycolysis were comparable for both genotypes when grown at 25 °C. However, there was a rapid global increase in the expression of most respiratory genes in response to chilling at 7 °C for both genotypes. When normally grown plant was set as the control for each genotype, the transcripts of mostCOXfamily members,ATP synthase,AOX1b, andUCPare highly up-regulated in chilling-tolerant Zhefen No. 208 plants in contrast to the sensitive Zhefen No. 212 plants. Both genotypes mobilized the energy-saving sucrose synthase pathway for sucrose degradation by cytosolic glycolysis, but this mechanism is evidently more effective in tolerant Zhefen No. 208 plants. Furthermore, only Zhefen No. 208 plants were able to partially switch from low-energy efficiency pathways to ATP conserving pathways to carry out fructose-6-phosphate conversion and pyruvate production. This metabolic flexibility in miETC and cytosolic glycolysis were coupled to higher ATP synthesis and lower ROS accumulation, which may be essential for sustaining the higher leaf respiration and homeostasis of chilling-tolerant plants.
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影响因子：
7.3
作者：
Armstrong, AF;Logan, DC;Atkin, OK
通讯作者：
Atkin, OK
DOI：
10.1093/emboj/16.16.4806
发表时间：
1997-08
期刊：
The EMBO Journal
影响因子：
--
作者：
H. Willekens;S. Chamnongpol;M. Davey;M. Schraudner;C. Langebartels;M. Van Montagu;D. Inzé;W. Van Camp-W.
通讯作者：
H. Willekens;S. Chamnongpol;M. Davey;M. Schraudner;C. Langebartels;M. Van Montagu;D. Inzé;W. Van Camp-W.
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
3.2
作者：
Patrick M. Finnegan;K. Soole;A. L. Umbach
通讯作者：
Patrick M. Finnegan;K. Soole;A. L. Umbach
影响因子：
6.4
作者：
Jia Wang;Nirusan Rajakulendran;S. Amirsadeghi;G. C. Vanlerberghe
通讯作者：
Jia Wang;Nirusan Rajakulendran;S. Amirsadeghi;G. C. Vanlerberghe