Decreased Expression of Transient Receptor Potential Vanilloid 1 Impairs the Postischemic Recovery of Diabetic Mouse Hearts
Decreased Expression of Transient Receptor Potential Vanilloid 1 Impairs the Postischemic Recovery of Diabetic Mouse Hearts
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瞬时受体电位 Vanilloid 1 表达减少会损害糖尿病小鼠心脏的缺血后恢复
DOI：
10.1253/circj.cj-08-0945
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发表时间：
2009-06-01
影响因子：
3.3
通讯作者：
Zheng, Liangrong
中科院分区：
文献类型：
--
作者：
Wei, Zhonghai;Wang, Lihong;Zheng, Liangrong
背景资料：我们假设，瞬时受体电位香草酸1型（TRPV 1），这是发现在感觉神经纤维，可以调节心脏功能，糖尿病受损，并可能导致进一步严重的postischemic心脏injuries.Methods和结果：糖尿病诱导ICR小鼠腹腔注射链脲佐菌素。糖尿病心脏TRPV 1和降钙素基因相关肽（CGRP）的表达均显著降低，分别通过Western blot和放射免疫法测定。在缺血/再灌注过程中，采用Medlab系统测定心功能，采用ELISA试剂盒测定流出液中的乳酸脱氢酶（LDH）。与非糖尿病心脏相比，糖尿病心脏左室舒张末期压升高，左室发展压、心率和冠脉流量降低，流出液中LDH释放量增加。辣椒素预处理可明显减轻非糖尿病心肌缺血后的损伤，但对糖尿病心肌缺血后的损伤无明显影响。结论：糖尿病引起的TRPV 1改变与心肌缺血后心功能恢复不良有关。(Circ J 2009; 73：1127-1132）
Background: We hypothesized that the transient receptor potential vanilloid type 1 (TRPV1), which is found in the sensory nerve fibers, could modulate the cardiac function, be impaired by diabetes and could contribute to further severe postischemic heart injury.Methods and Results: Diabetes was induced in ICR mice by an intraperitoneal injection of streptozotocin. The expression of both TRPV1 and calcitonin gene-related peptide (CGRP) in diabetes mellitus (DM) hearts was significantly lower, as determined by Western blot and radioimmunoassay, respectively. During the ischemia/reperfusion, the cardiac function was measured by a Medlab system and the lactate dehydrogenase (LDH) in the effluents was measured by an ELISA kit. Compared with the non-DM hearts, the DM hearts demonstrated increased left ventricular end-diastolic pressure and decreased left ventricular developed pressure, heart rate and coronary flow, and also released more LDH in the effluents. Pretreatment with capsaicin attenuated the postischemic injury significantly in non-DM hearts, but not in DM hearts. Pretreatment with CGRP in both non-DM and DM hearts protected hearts against ischemic injury.Conclusions: The alteration of TRPV1 caused by diabetes is related to the poor recovery of cardiac function after myocardial ischemia. (Circ J 2009; 73: 1127-1132)