ENZYMATIC-HYDROLYSIS OF BIOPOLYMERS VIA SINGLE-SCISSION ATTACK PATHWAYS - A UNIFIED KINETIC-MODEL
ENZYMATIC-HYDROLYSIS OF BIOPOLYMERS VIA SINGLE-SCISSION ATTACK PATHWAYS - A UNIFIED KINETIC-MODEL
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DOI：
10.1007/bf00701682
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发表时间：
1991-01-01
影响因子：
3.7
通讯作者：
LOTAN, N
中科院分区：
文献类型：
--
作者：
AZHARI, R;LOTAN, N
本文提出了一个统一的分析模型来描述聚合物酶水解的单断裂攻击途径。 该模型包括首选的攻击降级，以及内型和外型的。 该模型是基于动力学和统计的考虑。 它被开发来解释各种反应特性，包括动力学常数取决于聚合物基质的链长的情况。 分析模型提供了酶降解过程的动态描述，包括总反应速率、反应的时间过程和整个过程中获得的产物阵列。 这些特性被证明是受影响的，在很大程度上，即使是微小的变化，键断裂的概率。 该模型还提供了定量的证据，认为内型和外型降解途径，但一般情况下，首选的攻击之一的特殊情况。
A unified analytical model is suggested for describing single-scission attack pathways for enzymic hydrolysis of polymers. This model encompasses the preferred attack degradation, as well as the endo- and exo-type ones. The model is based on kinetic and statistic considerations. It was developed to account for a variety of reaction characteristics, including cases in which the kinetic constants depend upon the chain length of the polymeric substrate. The analytical model provides a dynamic description of the enzymic degradation process, including the overall reaction rate, the time course of the reaction and the array of products obtained throughout the process. These characteristics are shown to be affected, to a large extent, by even minor changes in the probabilities for bond cleavage. The model developed also provides the quantitative evidence for the view that the endo- and exo-type degradation pathways are but particular cases of the general, preferred attack one.