A NOVEL PUTATIVE G-PROTEIN-COUPLED RECEPTOR HIGHLY EXPRESSED IN LUNG AND TESTIS
A NOVEL PUTATIVE G-PROTEIN-COUPLED RECEPTOR HIGHLY EXPRESSED IN LUNG AND TESTIS
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DOI：
10.1089/dna.1993.12.393
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发表时间：
1993-06-01
影响因子：
3.1
通讯作者：
SPRENGEL, R
中科院分区：
文献类型：
--
作者：
EVA, C;SPRENGEL, R
通过聚合酶链式反应和常规文库筛选，获得了一个新的G蛋白偶联受体。用与甲状腺和G蛋白偶联受体之间保守的第六跨膜区相对应的简并引物，选择性地从神经母细胞瘤-胶质瘤细胞系中扩增出该受体家族新成员的基因片段。其中一个经序列分析似乎含有一个新的G蛋白偶联受体基因的部分片段的PCR扩增产物被用作探针，用于筛选大鼠前脑cDNA文库，以分离全长克隆。序列比较表明，该新基因属于G蛋白偶联受体家族，与狗RDC1孤儿受体和几种激素肽受体具有最高的结构相似性。这种假定的受体的mRNA不在大脑的任何特定区域表达，但似乎定位于有限数量的外围组织，包括肺、睾丸和肾脏。我们推测该克隆编码一种新的G蛋白偶联受体，该受体可能属于激素肽受体家族。
A putative novel G protein-coupled receptor was isolated using the polymerase chain reaction (PCR) and conventional library screening. Degenerate primers corresponding to the thyroid and the sixth transmembrane regions that are conserved among the G protein-coupled receptors were used to amplify selectively gene fragments of new members of this receptor family from a neuroblastoma-glioma cell line cDNA. One of the PCR amplification products that by sequence analysis appeared to contain a partial fragment of a novel G protein-coupled receptor gene was used as a probe to screen a rat forebrain cDNA library to isolate a full-length clone. Sequence comparison indicates that the new gene belongs to the G protein-coupled receptor family and displays the highest structural similarity with the dog RDC1 orphan receptor and several hormone peptide receptors. The mRNA for this putative receptor is not expressed in any specific region of the brain but seems to be localized in a restricted number of peripheral tissues, including lung, testes, and kidney. We suggest that the 4-24 cDNA clone encodes for a novel G protein-coupled receptor that may belong to hormone peptide receptor family.