Pax3/Pax7 mark a novel population of primitive myogenic cells during development
Pax3/Pax7 mark a novel population of primitive myogenic cells during development
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DOI：
10.1101/gad.345505
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发表时间：
2005-06-15
影响因子：
10.5
通讯作者：
Tajbakhsh, S
中科院分区：
文献类型：
--
作者：
Kassar-Duchossoy, L;Giacone, E;Tajbakhsh, S
骨骼肌作为一个范例的收购细胞的命运，但原始细胞群和新兴的成肌细胞之间的关系仍然难以捉摸。我们发现了一个新的居民Pax 3（+）/Pax 7（+），肌肉标记物阴性细胞在整个发展的人口。使用小鼠突变体，解偶联肌源性进展，我们表明，这些Pax（+）细胞产生肌肉祖细胞。在没有骨骼肌的情况下，它们在下调Pax 7后进行糖表达。此外，它们标志着胎儿发育期间卫星细胞的出现，并且不需要Pax 3功能。这些发现确定了谱系限制过程中的关键细胞群，并为治疗研究定义肌源性细胞状态提供了一个框架。
Skeletal muscle serves as a paradigm for the acquisition of cell fate, yet the relationship between primitive cell populations and emerging myoblasts has remained elusive. We identify a novel population of resident Pax3(+)/ Pax7(+), muscle marker-negative cells throughout development. Using mouse mutants that uncouple myogenic progression, we show that these Pax(+) cells give rise to muscle progenitors. in the absence of skeletal muscle, they apoptose after down-regulation of Pax7. Furthermore, they mark the emergence of satellite cells during fetal development, and do not require Pax3 function. These findings identify critical cell populations during lineage restriction, and provide a framework for defining myogenic cell states for therapeutic studies.