PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES IDENTIFYING BREAST TUMOR-ASSOCIATED ANTIGENS
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES IDENTIFYING BREAST TUMOR-ASSOCIATED ANTIGENS
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DOI：
10.1073/pnas.86.4.1362
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发表时间：
1989-02-01
影响因子：
11.1
通讯作者：
HIZI, A
中科院分区：
文献类型：
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作者：
KEYDAR, I;CHOU, CS;HIZI, A
我们制备了一种针对人乳腺腺癌细胞株T47D体外释放的逆转录病毒样颗粒（人乳腺肿瘤病毒）的小鼠单克隆抗体（H23）。该抗体与T47D细胞生长培养基和乳腺腺癌患者胸膜积液中检测到的表观分子质量为68 kDa （gp68）的糖蛋白特异性反应。在非乳腺癌患者的胸腔积液中未观察到这种抗原的水平。h23相关抗原定位于乳腺肿瘤细胞的细胞质以及乳腺癌患者的T47D细胞和转移细胞的细胞表面。在间接免疫过氧化物酶测定中使用H23对812例患者的组织进行了调查。结果显示，91%的乳腺肿瘤检测到抗原。正常组织和非乳腺癌组织均未见细胞质染色。在接受检测的56个乳腺组织样本中，只有一个对这种抗原呈阳性反应。鉴于该抗体特异性检测乳腺肿瘤细胞的能力，H23可能在诊断和患者的临床随访中具有重要意义，可以通过影像学检测转移性病变并进行治疗。
We have generated a mouse monoclonal antibody (H23) against the retrovirus-like particles (human mammary tumor virus) released in vitro by the human breast adenocarcinoma cell line T47D. This antibody reacts specifically with a glycoprotein with an apparent molecular mass of 68 kDa (gp68) that is detected in the growth medium of T47D cells as well as in pleural effusion fluids from breast adenocarcinoma patients. No detectable levels of this antigen could be observed in pleural effusions of patients with cancers other than of breast origin. The H23-related antigen was localized in the cytoplasm of breast tumor cells as well as on the cell surface of both T47D cells and metastatic cells from breast cancer patients. A survey of tissue from 812 patients was performed by using H23 in an indirect immunoperoxidase assay. The results showed that the antigen was detectable in 91% of all breast tumors tested. No cytoplasmic staining was observed in either normal tissues or nonbreast carcinomas. Only one of the benign breast tissues tested (out of a total of 56 samples of tissue) was positive for this antigen. Given the ability of this antibody to specifically detect breast tumor cells, H23 may be of importance in diagnosis and in clinical follow-up of patients for the detection of metastatic lesions by imaging and for therapy.