Multi-mycotoxin analysis of animal feed and animal-derived food using LC-MS/MS system with timed and highly selective reaction monitoring
Multi-mycotoxin analysis of animal feed and animal-derived food using LC-MS/MS system with timed and highly selective reaction monitoring
复制标题
使用 LC-MS/MS 系统对动物饲料和动物源性食品进行多种霉菌毒素分析，并进行定时和高选择性反应监测
DOI：
10.1007/s00216-015-8898-5
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Hou Jiafa
中科院分区：
文献类型：
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作者：
Zhao Zhiyong;Liu Na;Yang Lingchen;Deng Yifeng;Wang Jianhua;Song Suquan;Lin Shanhai;Wu Aibo;Zhou Zhenlei;Hou Jiafa
真菌毒素有可能通过将饲料中的污染物带入动物源性产品而进入人类食物链。本研究的目的是建立一种可靠和灵敏的方法，用于分析动物饲料和动物源性食品（肉类，可食用动物组织和牛奶）中的30种真菌毒素，使用液相色谱-串联质谱（LC-MS/MS）。在该研究中，评价了三种提取程序以及各种净化程序，以选择最适合不同样品基质的样品制备程序。此外，使用LC-MS/MS上的定时和高选择性反应监测来滤除同量异位素基质干扰。根据委员会决议2002/657/EC和401/2006/EC测定了该方法的性能特征（线性、灵敏度、回收率、精密度和专属性）。该方法已成功应用于饲料及动物源性食品中霉菌毒素的检测。结果表明，饲料中霉菌毒素污染直接影响动物源性食品中霉菌毒素的存在。图示摘要动物饲料和动物源性食品中多种霉菌毒素的LC-MS/MS分析
Mycotoxins have the potential to enter the human food chain through carry-over of contaminants from feed into animal-derived products. The objective of the study was to develop a reliable and sensitive method for the analysis of 30 mycotoxins in animal feed and animal-derived food (meat, edible animal tissues, and milk) using liquid chromatography–tandem mass spectrometry (LC–MS/MS). In the study, three extraction procedures, as well as various cleanup procedures, were evaluated to select the most suitable sample preparation procedure for different sample matrices. In addition, timed and highly selective reaction monitoring on LC–MS/MS was used to filter out isobaric matrix interferences. The performance characteristics (linearity, sensitivity, recovery, precision, and specificity) of the method were determined according to Commission Decision 2002/657/EC and 401/2006/EC. The established method was successfully applied to screening of mycotoxins in animal feed and animal-derived food. The results indicated that mycotoxin contamination in feed directly influenced the presence of mycotoxin in animal-derived food.Graphical abstractMulti-mycotoxin analysis of animal feed and animal-derived food using LC-MS/MS