Variation of cytosine methylation in response to water availability in two contrasting growth types of an amphibious plant Alternanthera philoxeroides
Variation of cytosine methylation in response to water availability in two contrasting growth types of an amphibious plant Alternanthera philoxeroides
复制标题
两栖植物空心莲子草两种不同生长类型中胞嘧啶甲基化对水分可用性的变化
DOI：
10.1016/j.bse.2013.03.053
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发表时间：
2013-10-01
影响因子：
1.6
通讯作者：
Chen, Jinming
中科院分区：
文献类型：
--
作者：
Li, Weiguo;Chen, Wenbo;Chen, Jinming
我们使用甲基化敏感的扩增多态性(MSAP)来检测两种不同生长类型的空心莲子草中胞嘧啶甲基化的变化，以响应水分供应的变化。利用34对选择性引物组合，在喜旱莲根和叶片中分别扩增出1026条和1128条清晰可重复的条带。当将水生植物移植到干旱培养中时，我们发现总体DNA甲基化水平有所下降。当陆地类型的植物被转移到洪水培养中时，我们检测到甲基化事件的频率高于去甲基化事件。根中DNA甲基化的变化比叶中更明显，对水分有效性的响应。剔除可变环境因素的混杂影响后，观察到在相同生长条件下，两种生长类型植物的胞嘧啶甲基化图谱存在差异。(C)2013爱思唯尔有限公司。保留所有权利。
We used methylation-sensitive amplified polymorphisms (MSAP) to examine the alterations of cytosine methylation in two contrasting growth types of an amphibious plant Alternanthera philoxeroides in response to change of water availability. Using 34 pairs of selective primer combinations, we amplified 1026 and 1128 clear and reproducible bands in root and leaf of A. philoxeroides, respectively. When the aquatic types of plants were transplanted into drought culture, we found a decrease in the overall DNA methylation. When the terrestrial types of plants were transferred into flood culture, we detected a higher frequency of methylation than demethylation events. Alterations of DNA methylation were more evident in root than in leaf in response to change of water availability. When the confounding effects of variable environmental factors were removed, differences of cytosine methylation profiles were observed between two growth types of plants under common growth conditions. (C) 2013 Elsevier Ltd. All rights reserved.