Cell volume change through water efflux impacts cell stiffness and stem cell fate
Cell volume change through water efflux impacts cell stiffness and stem cell fate
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DOI：
10.1073/pnas.1705179114
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发表时间：
2017-10-10
影响因子：
11.1
通讯作者：
Weitz, David A.
中科院分区：
文献类型：
--
作者：
Guo, Ming;Pegoraro, Adrian F.;Weitz, David A.
细胞改变其机械特性以响应其局部微环境;这在决定细胞功能中起作用，甚至可以影响干细胞的命运。在这里，我们确定了一个强大的和统一的细胞刚度和细胞体积之间的关系。当细胞在基底上扩散时，其体积减小，而其刚度随之增加。我们发现，皮质和细胞质细胞刚度规模与体积的许多扰动，包括不同的基板刚度，细胞扩散面积，和外部渗透压。细胞体积的减少是水流出的结果，其导致细胞内分子拥挤的相应增加。此外，我们发现，细胞体积的变化，从而刚度，改变干细胞分化，无论这些诱导的方法。这些观察结果揭示了细胞硬度和细胞体积之间令人惊讶的，以前未被识别的关系，强烈影响细胞生物学。
Cells alter their mechanical properties in response to their local microenvironment; this plays a role in determining cell function and can even influence stem cell fate. Here, we identify a robust and unified relationship between cell stiffness and cell volume. As a cell spreads on a substrate, its volume decreases, while its stiffness concomitantly increases. We find that both cortical and cytoplasmic cell stiffness scale with volume for numerous perturbations, including varying substrate stiffness, cell spread area, and external osmotic pressure. The reduction of cell volume is a result of water efflux, which leads to a corresponding increase in intracellular molecular crowding. Furthermore, we find that changes in cell volume, and hence stiffness, alter stem-cell differentiation, regardless of the method by which these are induced. These observations reveal a surprising, previously unidentified relationship between cell stiffness and cell volume that strongly influences cell biology.