Control of actin polymerization via reactive oxygen species generation using light or radiation.
Control of actin polymerization via reactive oxygen species generation using light or radiation.
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DOI：
10.3389/fcell.2022.1014008
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发表时间：
2022
影响因子：
5.5
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中科院分区：
文献类型：
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肌动蛋白是细胞中最普遍的蛋白质之一，其氨基酸序列从原生动物到人类都非常保守。质膜正下方肌动蛋白的聚合-解聚循环调节细胞功能、运动和形态。众所周知，肌动蛋白和其他肌动蛋白结合蛋白是活性氧 (ROS) 的靶标，表明 ROS 通过肌动蛋白重组影响细胞。一些研究人员试图从细胞外控制肌动蛋白聚合，以模拟或抑制肌动蛋白重组。据报道，为了改变肌动蛋白的聚合状态，紫外线、可见光和近红外光、电离辐射和发色团辅助光灭活都可以诱导ROS。此外，荧光蛋白KillerRed和发光蛋白荧光素酶的组合可以在肌动蛋白纤维上产生ROS并促进肌动蛋白聚合。这些技术是分析 ROS 与细胞功能、运动和形态之间关系的非常有用的工具，也有望用于治疗。在这篇小型综述中，我们概述了该领域的进展，特别关注如何使用此类光学方法控制细胞内肌动蛋白聚合，并讨论了未来的挑战。
Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility, and morphology. It is known that actin and other actin-binding proteins are targets for reactive oxygen species (ROS), indicating that ROS affects cells through actin reorganization. Several researchers have attempted to control actin polymerization from outside the cell to mimic or inhibit actin reorganization. To modify the polymerization state of actin, ultraviolet, visible, and near-infrared light, ionizing radiation, and chromophore-assisted light inactivation have all been reported to induce ROS. Additionally, a combination of the fluorescent protein KillerRed and the luminescent protein luciferase can generate ROS on actin fibers and promote actin polymerization. These techniques are very useful tools for analyzing the relationship between ROS and cell function, movement, and morphology, and are also expected to be used in therapeutics. In this mini review, we offer an overview of the advancements in this field, with a particular focus on how to control intracellular actin polymerization using such optical approaches, and discuss future challenges.