CYTO-TOXIC T-CELLS ARE INDUCED IN MICE INFECTED WITH LYMPHOCYTIC CHORIOMENINGITIS VIRUS-STRAINS OF MARKEDLY DIFFERENT PATHOGENICITIES
CYTO-TOXIC T-CELLS ARE INDUCED IN MICE INFECTED WITH LYMPHOCYTIC CHORIOMENINGITIS VIRUS-STRAINS OF MARKEDLY DIFFERENT PATHOGENICITIES
复制标题
DOI：
10.1128/iai.36.2.598-602.1982
复制
发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
PEVEAR, DC
中科院分区：
文献类型：
--
作者：
PFAU, CJ;VALENTI, JK;PEVEAR, DC
2 淋巴细胞性脉络膜脑膜炎病毒亚株在 i.c. 中诱导细胞毒性 T 淋巴细胞 (CTL) 反应的能力对受感染的小鼠进行了检查。一种被命名为 A（攻击性）的菌株在 8-9 天内引起 100% 的小鼠痉挛性死亡；另一种菌株，称为 D（温顺），在 28 天的观察期内杀死了 < 10% 的小鼠。通过部分纯化的脾细胞裂解感染任一类型淋巴细胞性脉络膜脑膜炎亚株的 51Cr 标记的 L 细胞靶标的能力来评估 CTL 活性。 CTL群体通过其对抗Thy-1血清的敏感性以及其不能裂解未感染的靶细胞或感染的靶细胞来鉴定，其与主要组织相容性基因复合物控制的抗原水平不同。在感染淋巴细胞性脉络膜脑膜炎亚株的小鼠中，产生了强烈的 CTL 反应，尽管 D 亚株引发的活性略低于 A 亚株感染后观察到的活性。两种菌株诱导的 CTL 活性峰值大约在同一时间发生。尽管 D 病毒在大脑中的复制范围比 A 病毒更广泛，并且荧光抗体染色显示病毒抗原的分布相似，但在感染 D 病毒的小鼠大脑中没有发现炎症反应。讨论了 CTL 在调节经典 CNS 病理学中的作用。
The ability of 2 lymphocytic choriomeningitis virus substrains to induce cytotoxic T lymphocyte (CTL) responses in i.c. infected mice was examined. One strain, designated A (aggressive), provoked a convulsive type of death in 100% of the mice within 8-9 days; the other strain, designated D (docile), killed < 10% of the mice during 28 day observation periods. CTL activity was assessed by the capacity of partially purified splenocytes to lyse 51Cr-labeled L cell targets infected with either type of lymphocytic choriomeningitis substrain. The CTL population was identified by its sensitivity to anti-Thy-1 serum and its inability to lyse uninfected target cells or infected target cells with which it differed at the level of antigens controlled by the major histocompatability gene complex. A strong CTL response developed in mice infected with either lymphocytic choriomeningitis substrain, although the activity provoked by substrain D was somewhat less than that seen after substrain A infection. Peak CTL activities induced by both strains occurred at about the same time. Even though D virus replicated more extensively in the brain than did A virus and fluorescent antibody staining revealed similar distributions of viral antigen, no inflammatory response was noted in the brains of mice infected with D virus. The role of CTL in mediating classic CNS pathology is discussed.