Arterial wall adaptation under elevated longitudinal stretch in organ culture
Arterial wall adaptation under elevated longitudinal stretch in organ culture
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DOI：
10.1114/1.1561291
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发表时间：
2003-04-01
影响因子：
3.8
通讯作者：
Vito, RP
中科院分区：
文献类型：
--
作者：
Han, HC;Ku, DN;Vito, RP
体内动脉受到较大的纵向拉伸，这种拉伸可能会因血管疾病和手术而发生显着变化。然而，尽管血压和血流引起的拉应力和剪应力的影响已得到充分记录，但人们对纵向拉伸对血管功能和血管壁重塑的影响知之甚少。为了研究纵向拉伸对血管功能和管壁重塑的影响，将猪颈动脉纵向拉伸比体内多20%，持续5天，同时在生理压力下的脉动流条件下维持在离体器官培养系统中。血管活力通过对去甲肾上腺素（NE，10（-6）M）、卡巴胆碱（10（-6）M）和硝普钠（10（-5）M）的强烈血管舒缩反应以及线粒体活性的密集染色和低细胞坏死发生率来证明。通过掺入溴脱氧尿苷（BrdU）来检查细胞增殖。结果显示，器官培养 5 天后，动脉仍保持正常结构和活力。拉伸动脉和控制动脉均表现出显着的收缩反应。例如，受 NE 影响，拉伸动脉和对照动脉均显示出约 10% 的直径收缩。拉伸的动脉含有 8% 的 BrdU 阳性细胞，而对照组的这一比例为 5% (p
Arteries in vivo are subjected to large longitudinal stretch which may change significantly due to vascular disease and surgery. However, little is known about the effect of longitudinal stretch on vascular function and wall remodeling, although the effects of tensile and shear stress from blood pressure and flow have been well documented. To study the effect of longitudinal stretch on vascular function and wall remodeling, porcine carotid arteries were longitudinally stretched 20% more than in vivo for 5 days while being maintained in an ex vivo organ culture system under conditions of pulsatile flow at physiologic pressure. Vessel viability was demonstrated by strong vasomotor responses to norepinephrine (NE, 10(-6) M), carbachol (10(-6) M), and sodium nitroprusside (10(-5) M), as well as by dense staining for mitochondrial activity and a low occurrence of cell necrosis. Cell proliferation was examined by incorporation of bromodeoxyuridine (BrdU). Results showed that arteries maintain normal structure and viability after 5 days in organ culture. Both the stretched and control arteries demonstrated significant contractile responses. For example, both stretched and control arteries showed approximately 10% diameter contraction in response to NE. Stretched arteries contained 8% BrdU-positive cells compared to 5% in controls (p