Marmoricola bigeumensis sp nov., a member of the family Nocardioidaceae
Marmoricola bigeumensis sp nov., a member of the family Nocardioidaceae
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DOI：
10.1099/ijs.0.65576-0
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Kim, Chang-Jin
中科院分区：
文献类型：
--
作者：
Dastager, Syed G.;Lee, Jae-Chan;Kim, Chang-Jin
从韩国Bigeum岛采集的土壤样品中分离到一株革兰氏阳性需氧菌，命名为MSL-05（T），并使用多相方法对其分类地位进行了研究。新菌株的细胞是球形的并且单独出现。新菌株在宽范围的NaCl浓度（0- 7%，w/v）下生长。最适生长温度和pH分别为28 ℃和7.2。化学分类学特征[肽聚糖类型，内消旋二氨基庚二酸;主要甲基萘醌，MK-8（H-4）;主要脂肪酸，i-C-16：0、ai-C-17：0、C-16：0和i-C-14：0; DNA G + C含量为72.9 mol%]将该微生物归入Marmoricola属。16 S rRNA基因序列分析证实了这一分类，但表明新菌株与其最近的邻居不同。菌株MSL-05（T）与Marmoricola属的模式菌株属于同一分支，与Marmoricola aequoreus JCM 13812（T）和Marmoricola aurantiacus DSM 12652（T）的基因序列相似性较低（96.91%和95.39%）。根据表型和系统发育分析结果，建议将该新分离物归类为Marmoricola属的一个新种，并建议将其命名为Marmoricola bigeumensis sp. nov.。模式菌株是MSL-05（T）（=KCTC 19287（T）=DSM 19426（T））。
A Gram-positive, aerobic strain, designated MSL-05(T), was isolated from a soil sample collected from Bigeum Island, Korea, and its taxonomic position was investigated using a polyphasic approach. Cells of the novel strain were coccoid and occurred singly. The novel strain grew over a wide range of NaCl concentrations (0-7%, w/v). The optimum growth temperature and pH were 28 degrees C and 7.2, respectively. Chemotaxonomic characteristics [peptidoglycan type, meso-diaminopimelic acid; major menaquinone, MK-8(H-4); predominant fatty acids, i-C-16:0, ai-C-17:0, C-16:0 and i-C-14:0; DNA G + C content of 72.9 mol%] placed this organism within the genus Marmoricola. 16S rRNA gene sequence analysis confirmed this classification, but showed that the novel strain was distinct from its nearest neighbours. Strain MSL-05(T) was found in the same clade as the type strains of species of the genus Marmoricola and also shared low gene sequence similarity with Marmoricola aequoreus JCM 13812(T) (96.91%) and Marmoricola aurantiacus DSM 12652(T) (95.39%). Based on the results of phenotypic and phylogenetic analyses, it is proposed that the new isolate should be classified as a novel species in the genus Marmoricola, for which the name Marmoricola bigeumensis sp. nov. is proposed. The type strain is MSL-05(T) (=KCTC 19287(T) =DSM 19426(T)).