QUANTITATION AND PROGNOSTIC VALUE OF BREAST-CANCER ANGIOGENESIS - COMPARISON OF MICROVESSEL DENSITY, CHALKLEY COUNT, AND COMPUTER IMAGE-ANALYSIS
QUANTITATION AND PROGNOSTIC VALUE OF BREAST-CANCER ANGIOGENESIS - COMPARISON OF MICROVESSEL DENSITY, CHALKLEY COUNT, AND COMPUTER IMAGE-ANALYSIS
复制标题
乳腺癌血管生成的定量和预后价值--微血管密度、查克利计数和计算机图像分析的比较
DOI：
10.1002/path.1711770310
复制
发表时间：
1995-11-01
影响因子：
7.3
通讯作者：
HARRIS, AL
中科院分区：
文献类型：
--
作者：
FOX, SB;LEEK, RD;HARRIS, AL
在一些乳腺癌研究中，免疫组织化学强调的微血管“热点”的定量已被证明是一种强大的预后工具。然而，所使用的抗体、评估的“热点”的数量和大小以及患者分为高血管组和低血管组的情况在不同的研究中有所不同。此外，微血管密度与其他血管参数之间的关系还知之甚少。这些不确定性和这项技术的繁琐性质使其不适合诊断实践。本研究采用手工和计算机图像分析技术，对30例浸润性乳腺癌不同大小的微血管密度与微血管参数之间的关系进行了研究。免疫组织化学染色的自动像素分析、Chalkley点计数和观察者主观血管分级也被评估为更快速地测量肿瘤血管的方法。对另外211例浸润性乳腺癌也进行了Chalkley计数。经计算机分析，手动微血管密度与管腔周长(r=0.60，P=0.0004)、管腔面积(r=0.56，P=0.002)、微血管数(r=0.57，P=0.0009)呈显著正相关。0.155 mm(2)和0.848 mm(2)的镜检热点与微血管数之间也存在显著的相关性(r=0.81，P
In some studies of breast cancer, quantitation of immunohistochemically highlighted microvessel 'hot spots' has been shown to be a powerful prognostic tool. However, the antibody used, the number and size of the 'hot spots' assessed, and the stratification of patients into high and low vascular groups vary between studies. Furthermore, little is known about the relationship between microvessel density and other vascular parameters. These uncertainties and the laborious nature of the technique make it unsuitable for diagnostic practice. Both manual and computerized image analysis techniques were used in this study to examine the relationship between microvessel density and the vascular parameters in different sized microscopic fields in a pilot series of 30 invasive breast carcinomas. Automated pixel analysis of immunohistochemical staining, Chalkley point counting, and observer subjective vascular grading were also assessed as more rapid methods of measuring tumour vascularity. A Chalkley count was also performed on a further 211 invasive breast carcinomas. Significant correlations were observed between manual microvessel density and luminal perimeter (r=0.6, P=0.0004), luminal area (r=0.56, P=0.002), and microvessel number (r=0.57, P=0.0009) by computerized analysis. There were also significant correlations between the microscopic hot spots of 0.155 mm(2) and 0.848 mm(2) for microvessel number (r=0.81, P