PLASMA-CLEARANCE, ORGAN DISTRIBUTION AND TARGET-CELLS OF INTERLEUKIN-6 HEPATOCYTE-STIMULATING FACTOR IN THE RAT
PLASMA-CLEARANCE, ORGAN DISTRIBUTION AND TARGET-CELLS OF INTERLEUKIN-6 HEPATOCYTE-STIMULATING FACTOR IN THE RAT
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DOI：
10.1111/j.1432-1033.1988.tb14384.x
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发表时间：
1988-11-01
期刊：
影响因子：
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通讯作者：
HEINRICH, PC
中科院分区：
文献类型：
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作者：
CASTELL, JV;GEIGER, T;HEINRICH, PC
通过测定125 I-rhIL-6的生物学活性和放射性从循环中的消失，测定了重组人白细胞介素-6（rhIL-6）在大鼠体内的血浆半衰期。清除动力学为双相。它由对应于3分钟半衰期的快速初始消失和对应于约55分钟半衰期的第二缓慢消失组成。通过醋酸纤维素电泳显示rhIL-6与血浆蛋白结合，导致复合物在β-葡萄糖中迁移。γ的静脉注射125 I-rhIL-6后20 min，约80%的125 I-rhIL-6从循环中消失，在肝脏中被发现。125 I-rhIL-6仅定位于肝实质细胞表面，表明肝细胞上存在白细胞介素-6受体。
The plasma half-life of recombinant human interleukin-6 (rhIL-6) was determined in rats by measuring the disappearance of the biological activity as well as of the radioactivity of 125I-rhIL-6 from the circulation. The kinetics of clearance were biphasic. It consisted of a rapid initial disappearance corresponding to a half-life of 3 min, and of a second slow one corresponding to a half-life of about 55 min. By cellulose-acetate electrophoresis it was shown that rhIL-6 binds to a plasma protein resulting in a complex migrating in the .beta.-.gamma. region; 20 min after intravenous injection, about 80% of the 125I-rhIL-6 that had disappeared from the circulation was found in the liver. 125I-rhIL-6 was exclusively localized on the surface of parenchymal cells suggesting the existence of an interleukin-6 receptor on the hepatocytes.