Spatially and temporally resolved calcium concentration changes in oscillating neurons of crab stomatogastric ganglion.
Spatially and temporally resolved calcium concentration changes in oscillating neurons of crab stomatogastric ganglion.
复制标题
蟹口胃神经节振荡神经元的空间和时间分辨钙浓度变化。
DOI：
10.1073/pnas.86.5.1679
复制
发表时间：
1989
影响因子：
11.1
通讯作者：
Graubard,K
中科院分区：
文献类型：
--
作者：
Ross,WN;Graubard,K
用钙指示剂偶氮砷III和光电二极管阵列在多个位置监测了蟹口胃神经元膜电位振荡过程中钙离子浓度的变化。开发了使用信号平均技术的数据分析算法，以提高测量的钙变化的时间分辨率。正如以前报道的那样，钙振荡在神经纤维的所有区域都被检测到，但在胞体或轴突中没有检测到。在一些细胞中，细胞内神经纤维膜钙的阶跃增加与爆发中的每个动作电位(在电压振荡的峰值上)相关。在其他细胞中，我们观察到钙振荡被锁定在膜电位上，没有尖峰相关成分。少数细胞既有尖峰诱发的电位成分，也有钙振荡的分级电位成分。在这些细胞中，与棘波相关的钙离子内流的空间分布与电压振荡相关的钙离子内流的空间分布不同，这表明不同的轴突可能通过不同的递质释放和棘波相关递质释放的不同混合物与其突触后靶相互作用。
Calcium concentration changes during oscillations of the membrane potential of crab (Cancer irroratus or Cancer borealis) stomatogastric neurons were monitored at many positions by using the calcium indicator dye arsenazo III and a photodiode array. Data analysis algorithms using signal averaging techniques were developed to improve the time resolution of the measured calcium changes. As previously reported, calcium oscillations were detected from all regions of the neuropil but not from the soma or axon. In some cells step increases in intracellular neuropil calcium were correlated with each of the action potentials in the burst (on the peak of the voltage oscillation). In other cells we observed calcium oscillations phase-locked to the membrane potential with no spike-related component. A few cells had both spike-evoked and graded potential components to the calcium oscillations. In those cells, the spatial distribution of the spike-correlated calcium influx differed from that of the voltage-oscillation-correlated calcium influx, suggesting that different neurites might interact with their postsynaptic targets with different mixtures of graded and spike-correlated transmitter release.