Authentication of Caulis clematidis Armandii (Chuanmutong) and differentiation of its common adulterants using RAPD and SCAR markers.
Authentication of Caulis clematidis Armandii (Chuanmutong) and differentiation of its common adulterants using RAPD and SCAR markers.
复制标题
使用 RAPD 和 SCAR 标记鉴定川木通及其常见掺假品。
DOI：
10.5897/jmpr10.074
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发表时间：
2010
影响因子：
--
通讯作者：
De
中科院分区：
文献类型：
--
作者：
Jin;Yan Ren;Lu Chen;Jin Pei;De
Caulis clematidis Armandii (“Chuanmutong” in Chinese) has been used as an important traditional Chinese herb for a long time. As recorded, the origins ofCaulis clematidis Armandii are the dried stems of Clematis armandii Franch.and C. montana Buch. -Ham. But due to the similarity in morphology, it is difficult to distinguish C. armandii and C. montana from other Clematis species. As a result, some Clematis species were mistakenly used asCaulis clematidis Armandii, and caused potentially dangerous consequences.To overcome the limitation of the traditional morphological methods, an efficientmolecular method was developed to better distinguishCaulis clematidis Armandii from its common adulterants. Two random amplified polymorphic DNA (RAPD) fragments specific for C. armandii and C. montanawere sequenced and primers were designed for sequence characterized amplified region (SCAR) markers. The two obtained SCAR markers were specific to both C. armandii and C. montana, but were absent from otherClematis species. The protocol developed in this study can be further applied to rapid authentication of Caulis Clematidis Armandii and its common adulterants.
Key words: Athentication, Caulis clematidis armandii, Clematis armandii,Clematis Montana, RAPD, SCAR.
基于应变测量的PC钢材料蠕变系数评估方法
DOI：
--
发表时间：
2013
期刊：
影响因子：
--
作者：
小川由布子;亀田昭一;佐藤良一;上谷康晴
通讯作者：
上谷康晴