Prenatal Exposure to Per- and Polyfluoroalkyl Substances (PFASs) and Association between the Placental Transfer Efficiencies and Dissociation Constant of Serum Proteins-PFAS Complexes
Prenatal Exposure to Per- and Polyfluoroalkyl Substances (PFASs) and Association between the Placental Transfer Efficiencies and Dissociation Constant of Serum Proteins-PFAS Complexes
复制标题
产前接触全氟烷基物质和多氟烷基物质 (PFAS) 以及胎盘转移效率与血清蛋白-PFAS 复合物解离常数之间的关联
DOI：
10.1021/acs.est.9b00715
复制
发表时间：
2019-06-04
影响因子：
11.4
通讯作者：
Jiang, Guibin
中科院分区：
文献类型：
--
作者：
Gao, Ke;Zhuang, Taifeng;Jiang, Guibin
关于短链全氟烷基和多氟烷基物质（PFAS）的胎盘转移和不良结局的信息有限，PFAS胎盘转移的因素仍不清楚。本研究分析了北京地区132例孕妇血清和脐带血中21种PFAS的浓度，并计算了每种PFAS的胎盘转运效率（PTE）。首次报道了短链全氟烷基酸（PFBA，146%），PFBS（97%），PFPeA（118%）和PFHxA（110%）的质子交换效率，并得到了全氟烷基羧酸（PFCAs）的质子交换效率从C4到C13的完整U型变化趋势.母体体重与全氟辛烷磺酸（PFOS）的PTE呈正相关（p < 0.05），母体PFBA浓度与出生身长呈负相关（p < 0.01）。通过体外实验进一步测定了人血清白蛋白（HSA）与PFAS复合物（Kd-Hp）、血清蛋白PFAS复合物（Kd-sp）和肝脂肪酸结合蛋白（L-FABP）与PFAS复合物（Kd-Lp）的平衡解离常数（K（d）s），发现它们与PFAS的PTE均呈显著相关。相关系数分别为0.92、0.89和0.86（均P < 0.01），提示蛋白（血清）-PFAS复合物的K（d）s在PFAS经胎盘转运中起重要作用，其中Kd-Hp起关键作用。
Information on placental transfer and adverse outcomes of short-chain per- and polyfluoroalkyl substance (PFASs) is limited, and factors responsible for PFAS placental transfer are still unclear. In the present study, concentrations of 21 PFASs were analyzed in 132 paired maternal and cord serum samples collected from residents in Beijing, China, and the placental transfer efficiency (PTE) of each PFAS was calculated. PTEs of short-chain perfluoroalkyl acids (PFAAs), including PFBA (146%), PFBS (97%), PFPeA (118%), and PFHxA (110%), were first reported, and a complete U-shaped trend of PTEs from C4 to C13 of perfluoroalkyl carboxylic acids (PFCAs) was obtained. Positive association between maternal weight and PTE of perfluorooctanesulfonate (PFOS) (p < 0.05) and negative association between maternal PFBA concentration and birth length (p < 0.01) were observed. Using in vitro experiments, we further determined equilibrium dissociation constants (K(d)s) of human serum albumin (HSA) PFAS complexes (Kd-Hp), serum proteins PFAS complexes (Kd-sp), and liver-fatty acid binding protein (L-FABP)-PFAS complexes (Kd-Lp) and found that they were all significantly correlated with PTEs of PFASs. The correlation coefficient was 0.92, 0.89, and 0.86, respectively (p < 0.01 in all three tests), suggesting that K(d)s of protein (serum)-PFAS complexes can play an important role in trans-placental transfer of PFASs in human and Kd-Hp plays a pivotal role.