Genetic diversity and insecticide. resistance of Myzus persicae (Hemiptera: Aphididae) populations from tobacco in Chile:: evidence for the existence of a single predominant clone
Genetic diversity and insecticide. resistance of Myzus persicae (Hemiptera: Aphididae) populations from tobacco in Chile:: evidence for the existence of a single predominant clone
复制标题
DOI：
10.1079/ber2003275
复制
发表时间：
2004-02-01
影响因子：
1.9
通讯作者：
Niemeyer, HM
中科院分区：
文献类型：
--
作者：
Fuentes-Contreras, E;Figueroa, CC;Niemeyer, HM
烟蚜Myzuspersicae（Sulzer）的食烟小种，以前称为M.烟草Blackman，在过去十年中被引入智利。为了评价智利烟蚜种群的遗传多样性和抗药性状况，在35个烟田进行了田间调查，覆盖300公里的纬度调查。采用微卫星标记和形态计量学多变量分析对采样的人群进行了表征。通过微孔板酯酶试验和kdr基因突变状态评估了对杀虫剂的抗性水平。所有采集的样品都对应于同一个非全周期蚜虫基因型，并且在M.桃蚜。酯酶活性显示的水平和变异性预期的R1克隆缺乏突变的钠通道（敏感的kdr），因此对应于一种类型的有机磷和氨基甲酸酯，并敏感拟除虫菊酯类杀虫剂。
The tobacco-feeding race of Myzus persicae (Sulzer), formerly known as M. nicotianae Blackman, was introduced into Chile during the last decade. In order to evaluate the genetic diversity and insecticide resistance status of Chilean tobacco aphid populations, a field survey was conducted in 35 tobacco fields covering a 300 kin latitudinal survey. The populations sampled were characterized using microsatellite markers and morphometric multivariate analysis. Insecticide resistance levels were assessed through a microplate esterase assay and the mutation status of the kdr gene. All samples collected corresponded to the same anholocyclic aphid genotype, and showed morphological variation within the range expected for the tobacco-feeding race of M. persicae. Esterase activity showed the level and variability expected for an R1 clone lacking mutations in the sodium channels (susceptible kdr), thus corresponding to a type slightly resistant to organophosphate and carbamate, and susceptible to pyrethroid insecticides.