NON-INVASIVE EVALUATION OF NERVE CONDUCTION IN SMALL DIAMETER FIBERS IN THE RAT.
NON-INVASIVE EVALUATION OF NERVE CONDUCTION IN SMALL DIAMETER FIBERS IN THE RAT.
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DOI：
10.1155/2013/254789
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发表时间：
2013-02
期刊：
影响因子：
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通讯作者：
E. Zotova;J. Arezzo
中科院分区：
文献类型：
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作者：
E. Zotova;J. Arezzo
应用一种新的非侵入性技术来测量坐骨神经远端中慢传导轴突内的速度（即，指神经）。该技术是基于从体内全神经复合反应中提取整流多单位活动（MUA）。该方法可靠地识别了以9-18 m/s范围传导的薄髓鞘纤维（Aδ轴突）以及以约1-2 m/s传导的未髓鞘化C纤维亚组中的复合动作电位。该方法对C-纤维传导的灵敏度通过在局部施用辣椒素后20天内在1-2 m/s范围内的反应的逐渐减少来证实（ANOVA p<0.03）。在0.75 Hz至6.0 Hz的范围内增加施加的重复刺激的频率，导致传导减慢和复合C纤维反应的幅度显著降低（ANOVA p<0.01）。该技术提供了一个独特的机会，非侵入性，可重复的，定量评估的速度在Aδ和C纤维的子集与快速神经传导的评价平行。
A novel non-invasive technique was applied to measure velocity within slow conducting axons in the distal extreme of the sciatic nerve (i.e., digital nerve) in a rat model. The technique is based on the extraction of rectified multiple unit activity (MUA) from in vivo whole nerve compound responses. This method reliably identifies compound action potentials in thinly myelinated fibers conducting at a range of 9-18 m/s (Aδ axons), as well as in a subgroup of unmylinated C fibers conducting at approximately 1-2 m/s. The sensitivity of the method to C-fiber conduction was confirmed by the progressive decrement of the responses in the 1-2 m/s range over a 20-day period following the topical application of capsaicin (ANOVA p<0.03). Increasing the frequency of applied repetitive stimulation over a range of 0.75 Hz to 6.0 Hz produced slowing of conduction and a significant decrease in the magnitude of the compound C-fiber response (ANOVA p<0.01). This technique offers a unique opportunity for the non-invasive, repeatable, and quantitative assessment of velocity in the subsets of Aδ and C fibers in parallel with evaluation of fast nerve conduction.