Serum from monkeys with histories of fetal wastage causes abnormalities in cultured rat embryos.
Serum from monkeys with histories of fetal wastage causes abnormalities in cultured rat embryos.
复制标题
来自有胎废史的猴子的血清会导致培养的大鼠胚胎异常。
DOI：
10.1126/science.7053560
复制
发表时间：
1982
期刊：
影响因子：
56.9
通讯作者：
R. Parker
中科院分区：
文献类型：
--
作者：
N. W. Klein;J. Plenefisch;S. W. Carey;W. T. Fredrickson;G. Sackett;T. Burbacher;R. Parker
将处于头褶皱中期的大鼠胚胎在雌性猴血清中培养48小时。根据胚胎反应，研究人员在不了解供体生殖史的情况下，正确地鉴定了18只恒河猴中2只高风险饲养员的血清和26只猪尾猴中14只高风险饲养员中12只的血清具有致畸性。胚胎反应参数检查，异常类型和频率更密切相关的供体生殖史比胚胎蛋白质含量或体节数。
Rat embryos in the mid-head-fold stage were cultured on female monkey serum for 48 hours. On the basis of embryo response, the investigators, with no knowledge of the donors' reproductive histories, correctly identified the serum from two high-risk breeders among 18 rhesus monkeys and the serum from 12 of 14 high-risk breeders among 26 pig-tailed monkeys as teratogenic. Of the embryo response parameters examined, abnormality type and frequency were more closely correlated with donor reproductive histories than embryo protein content or somite number.