Expression of RUSH transcription factors in developing and adult rabbit gonads
Expression of RUSH transcription factors in developing and adult rabbit gonads
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DOI：
10.1095/biolreprod63.1.156
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发表时间：
2000-07-01
影响因子：
3.6
通讯作者：
Lee, VH
中科院分区：
文献类型：
--
作者：
Rendon, A;Hewetson, A;Lee, VH
RUSH转录因子1 α和1 β与兔子宫珠蛋白启动子结合，是SWI/SNF复合物的成员，通过重塑染色质（解旋酶）促进转录。为了表征RUSH的性腺表达，将识别两种亚型的cRNA探针用于原位杂交研究。我们发现RUSH mRNA是丰富的支持细胞从胚胎，新生儿，青春期前，和青春期兔睾丸。在成年人中，RUSH mRNA在小管中检测到，小管中有细线期前的精母细胞和成熟的精子细胞排列在管腔中。RUSH mRNA在卵泡形成前的胚胎和新生期卵巢颗粒细胞中表达，在青春期前和成年期卵巢腔前卵泡周围的颗粒细胞和卵泡膜细胞中表达。RUSH在成熟卵巢有腔卵泡颗粒细胞中的表达仍然很高，但在晚期闭锁卵泡和黄体中的表达可以忽略不计。Western blot分析证实RUSH-1a亚型在睾丸和卵巢组织中占主导地位。RUSH的表达模式表明在支持细胞中以及在颗粒细胞分化的多个阶段中的转录活性，特别是在迄今为止被认为是休眠的原始卵泡中。
The RUSH transcription factors 1 alpha and 1 beta bind to the Rabbit Uteroglobin promoter and are members of the SWI/SNF complex that facilitates transcription by remodeling chromatin (Helicase). To characterize gonadal expression of RUSH, a cRNA probe that recognizes both isoforms was used for in situ hybridization studies. We found RUSH mRNA to be abundant in Sertoli cells from embryonic, neonatal, prepubertal, and pubertal rabbit testes. In adults, RUSH mRNA was detected in tubules with preleptotene spermatocytes and mature spermatids lining the lumen. However, RUSH was undetectable in tubules that contained leptotene spermatocytes and that lacked mature spermatids, In females, RUSH was expressed in presumptive granulosa cells of embryonic and neonatal ovaries before follicle organization, abundant RUSH mRNA was detected in granulosa and theca cells surrounding preantral follicles of prepubertal and adult ovaries. Expression of RUSH remained high in granulosa cells of antral follicles in mature ovaries but was negligible in late-stage atretic follicles and in corpora lutea. Western blot analysis confirmed the RUSH-1a isoform predominated in both testicular and ovarian tissues. The expression pattern of RUSH indicates transcriptional activity in Sertoli cells and during multiple stages of differentiating granulosa cells, especially those of primordial follicles, which heretofore were considered to be dormant.