DC-Dielectrophoretic separation of biological cells by size
DC-Dielectrophoretic separation of biological cells by size
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DOI：
10.1007/s10544-007-9130-y
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发表时间：
2008-04-01
影响因子：
2.8
通讯作者：
Eid, Josiane E.
中科院分区：
文献类型：
--
作者：
Kang, Yuejun;Li, Dongqing;Eid, Josiane E.
DC- dielectrophoresis （DC- dep）是一种利用介电粒子在空间非均匀直流电场中的诱导运动来分离生物细胞的方法。局部非均匀电场是由在PDMS微通道内制作的绝缘栅产生的。细胞在障碍的角落处经历负的DEP（相应地是斥力）力，那里的局部电场强度梯度最强。作用在电池上的DC-DEP力与电池的大小成正比。因此，移动的单元偏离流线，偏离的程度取决于单元的大小。在本文中，我们通过这种方法证明，结合电渗透流，可以将直径相差几微米到几十微米的混合生物细胞连续分离到不同的收集孔中。为了分离特定尺寸的靶细胞，所需要做的就是调整电极的电压输出。
DC-Dielectrophoresis (DC-DEP), the induced motion of the dielectric particles in a spatially non-uniform DC electric field, is applied to separate biological cells by size. The locally non-uniform electric field is generated by an insulating hurdle fabricated within a PDMS microchannel. The cells experience a negative DEP (accordingly a repulsive) force at the corners of the hurdle where the gradient of local electric-field strength is the strongest. The DC-DEP force acting on the cells is proportional to the cells' size. Thus the moving cells deviate from the streamlines and the degree of deviation is dependent on the cell size. In this paper, we demonstrated by using this method that, combined with the electroosmotic flow, mixed biological cells of a few to tens of micrometers difference in diameter can be continuously separated into different collecting wells. For separating target cells of a specific size, all that is required is to adjust the voltage outputs of the electrodes.