Role of arginine in superficial wound healing in man
Role of arginine in superficial wound healing in man
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DOI：
10.1016/j.niox.2009.07.006
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发表时间：
2009-11-01
影响因子：
3.9
通讯作者：
van der Hulst, R. R. W. J.
中科院分区：
文献类型：
--
作者：
Debats, I. B. J. G.;Wolfs, T. G. A. M.;van der Hulst, R. R. W. J.
精氨酸补充剂已被确定为有利于实验性伤口愈合。然而，组织修复中这种有益作用的机制仍然没有得到解决。动物研究表明，精氨酸补充剂的有益作用是介导的，至少部分是通过NO。后一种成分介导参与组织修复的过程，包括血管生成，上皮形成和胶原蛋白形成。这项前瞻性研究是为了调查精氨酸代谢在急性手术伤口在man. Expression的酶，已知参与精氨酸代谢，进行了研究，在供皮区的10名住院患者接受皮肤移植。使用高效液相色谱法测量精氨酸代谢物（鸟氨酸、瓜氨酸、硝酸盐和亚硝酸盐= NOx）的血浆和伤口液水平。采用免疫组织化学方法检测皮肤组织中iNOS、eNOS、iNOS-1和iNOS-2的表达。使用ELISA测量血浆和伤口液中的精氨酸酶-1浓度。精氨酸酶-2用蛋白质印迹法测定。与血浆相比，我们观察到伤口液中瓜氨酸、鸟氨酸、NOx和谷胱甘肽酶-1水平升高。精氨酸酶-2在血浆和伤口液中均表达，并且在血浆中似乎更高。在创伤后，中性粒细胞、巨噬细胞、成纤维细胞、角质形成细胞和内皮细胞表达iNOS，而内皮细胞和成纤维细胞中观察到eNOS反应性。精氨酸酶-1在创伤后中性粒细胞中表达，而精氨酸酶-2在内皮细胞、角质形成细胞、成纤维细胞、巨噬细胞和中性粒细胞中观察到染色。第一次，人类数据支持以前的动物研究表明精氨酸代谢的NO-以及辅酶Ⅱ酶介导的修复受伤的皮肤。(C)2009 Elsevier Inc. All rights reserved.
Arginine supplementation has been identified as advantageous in experimental wound healing. However, the mechanisms underlying this beneficial effect in tissue repair remain unresolved. Animal studies suggest that the beneficial role of arginine supplementation is mediated, at least in part through NO. The latter component mediates processes involved in tissue repair, including angiogenesis, epithelialization and collagen formation. This prospective study is performed to investigate arginine metabolism in acute surgical wounds in man. Expression of enzymes, known to be involved in arginine metabolism, was studied in donor sites of skin grafts of 10 hospitalized patients undergoing skin transplantation. Plasma and wound fluid levels of arginine metabolites (ornithine, citrulline, nitrate and nitrite = NOx) were measured using High Performance Liquid Chromatography. Expression of iNOS, eNOS, arginase-1 and arginase-2 was studied by immunohistochemistry in paraffin sections of skin tissue. Arginase-1 concentration was measured in plasma and wound fluid using ELISA. Arginase-2 was determined using Western blot analysis. We observed increased levels of citrulline, ornithine, NOx and arginase-1 in wound fluid when compared with plasma. Arginase-2 was expressed in both plasma and wound fluid and seemed higher in plasma. iNOS was expressed by neutrophils, macrophages, fibroblasts, keratinocytes and endothelial cells upon wounding, whereas eNOS reactivity was observed in endothelial cells and fibroblasts. Arginase-1 was expressed in neutrophils post-wounding, while arginase-2 staining was observed in endothelial cells, keratinocytes, fibroblasts, macrophages and neutrophils. For the first time, human data support previous animal studies suggesting arginine metabolism for an NO- as well as arginase-mediated reparation of injured skin. (C) 2009 Elsevier Inc. All rights reserved.