Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats' colon via NF-κB pathway.
Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats' colon via NF-κB pathway.
复制标题
肠易激综合症大鼠结肠的含水通道蛋白1、3和8通过NF-κB途径表达。
DOI：
10.18632/oncotarget.17565
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发表时间：
2017-07-18
期刊：
影响因子：
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通讯作者：
Zhang S
中科院分区：
文献类型：
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作者：
Chao G;Zhang S
本研究旨在检测水通道蛋白的表达。目的研究NF-κB在肠易激综合征（IBS）大鼠模型结肠中的表达，以期发现IBS新的发病机制。与对照组相比，IBS模型组AQP 1、AQP 3、AQP 8表达下调，NF-κB p65表达上调（p < 0.05）;与IBS模型组相比，抑制剂组AQP 1、AQP 3、AQP 8表达上调，NF-κB p65表达下调（p < 0.05）。将18只成年雌性SD大鼠随机分为正常对照组、IBS模型组和抑制剂组，每组18只，分别给予NF-κB抑制剂（PDTC）注射。采用免疫组化和western blot检测AQP 1、AQP 3、AQP 8和NF-κB p65的表达。RT-PCR检测AQP 1、AQP 3和AQP 8的表达。肠易激综合征的发病机制可能是通过NF-κB途径下调AQP 1、AQP 3和AQP 8的表达而引起的。
Our research was to detect the expression of aquaporins. NF-κB in Irritable bowel syndrome (IBS) rat models’ colon so as to find novel pathogenesisof IBS. The expression of AQP1, AQP3, and AQP8 of IBS model group was down-regulated while NF-κB p65 was up-regulated comparing with control group (p < 0.05), and the expression of AQP1, AQP3, and AQP8 of inhibitor group was up-regulated while NF-κB p65 was down-regulated comparing with IBS model group (p < 0.05). 18 adult female SD big rats were divided into three groups:the rats in control group were normal rats, the rats in IBS model group and the rats of inhibitor group were injected with the inhibitor of NF-κB (PDTC). Immunohistochemical technique and western blot were performed to detect the expression of AQP1, AQP3, AQP8 and NF-κB p65. RT-PCR was performed to detect the expression of AQP1, AQP3, and AQP8. Liquid water metabolic abnormalities and intestine permeability alteration might be the mechanism of IBS by down-regulating AQP1, AQP3 and AQP8 via NF-κB pathway.