Blockade of oxidative stress by vitamin C ameliorates albuminuria and renal sclerosis in experimental diabetic rats.
Blockade of oxidative stress by vitamin C ameliorates albuminuria and renal sclerosis in experimental diabetic rats.
复制标题
维生素C封闭氧化应激会改善实验性糖尿病大鼠的蛋白尿和肾硬化。
DOI：
10.3349/ymj.2007.48.5.847
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发表时间：
2007-10-31
影响因子：
2.4
通讯作者：
Hong SY
中科院分区：
文献类型：
--
作者：
Lee EY;Lee MY;Hong SW;Chung CH;Hong SY
氧化应激被认为是糖尿病中细胞凋亡和肾脏损伤的常见介质。然而，糖尿病病程中肾脏是否发生细胞凋亡尚不清楚。我们研究了糖尿病大鼠肾脏细胞凋亡的发生、氧化应激的作用以及抗氧化剂对糖尿病大鼠肾脏细胞凋亡的影响。 Otsuka-Long-Evans-Tokushima-Fatty 大鼠是 2 型糖尿病的动物模型，被随机分为未治疗的糖尿病组 (n = 8) 和维生素 C 治疗组 (n = 8)。 Long-Evans德岛大冢大鼠(n=8)用作对照。糖尿病大鼠的近曲小管上皮细胞中存在细胞凋亡。与未治疗的糖尿病大鼠相比，维生素 C 治疗的糖尿病大鼠的凋亡细胞数、白蛋白尿、蛋白尿、肾小球和肾小管间质硬化以及肾丙二醛显着减少。通过电子显微镜评估，减少的狭缝孔密度（每根肾小球基底膜长度的狭缝孔数量）也可以通过维生素 C 治疗得到显着恢复，而不会显着影响糖尿病大鼠的血浆葡萄糖。通过阻断这些病理生理过程，维生素 C 阻断氧化应激可能成为糖尿病肾病白蛋白尿和肾硬化的有用辅助手段。
Oxidative stress has been suggested to play a role as a common mediator of apoptosis and kidney damage in diabetes. However, it is uncertain whether the apoptosis occurs in the kidney during the course of diabetes. We investigated the occurrence of apoptosis in the diabetic rat kidney, the role of oxidative stress and the effect of an antioxidant on apoptosis in the diabetic rat kidney. Otsuka-Long-Evans-Tokushima-Fatty rats, an animal model for type 2 diabetes, were randomized into a non-treated diabetic (n = 8) and a vitamin C-treated group (n = 8). Long-Evans Tokushima Otsuka rats (n = 8 ) were used as a control. Apoptosis was present in the epithelial cells of the proximal tubules in diabetic rats. The number of apoptotic cells, albuminuria, proteinuria, glomerular and tubulointerstitial sclerosis, and renal malondialdehyde were significantly decreased in vitamin C-treated diabetic rats when compared to the untreated diabetic rats. The decreased slit pore density (number of slit pores per underlying glomerular basement membrane length) as assessed by electron microscopy was also significantly restored by treatment with vitamin C without significantly affecting plasma glucose in diabetic rats. By blocking these pathophysiologic processes, a blockade of oxidative stress by vitamin C might become a useful adjunct to albuminuria and renal sclerosis in diabetic nephropathy.